NovaSeq 6000 Xp Workflow

NovaSeq 6000 Xp Workflow delivers the flexibility of independent lane loading and high library multiplexing per flow cell, maximizing sample throughput on a single run.

NovaSeq 6000 Xp Workflow

NovaSeq 6000 Xp Workflow delivers the flexibility of independent lane loading and high library multiplexing per flow cell, maximizing sample throughput on a single run.

Overview

The NovaSeq 6000 Xp Workflow supports a variety of applications, such as whole-genome sequencing (WGS), ultradeep exome sequencing, and single-cell sequencing. Improving upon the standard workflow, the NovaSeq 6000 Xp Workflow adds a level of flexibility and control to experimental planning and flow cell configuration on the NovaSeq 6000 System.

Key features

  • Separation of projects or methods by lane

  • Ability to run incompatible library types on the same flow cell

  • Multiplexing within each lane to maximize total number of samples per flow cell

  • Lower concentration of denatured libraries 

Maximize flexibility and efficiency

The NovaSeq 6000 Xp Workflow offers the flexibility to load each flow cell lane independently and multiplex samples within each lane. These features maximize the total number of samples and reduce the required DNA input per flow cell without compromising data quality or yield.

Specifications

Instruments NovaSeq 6000 System
Maximum output 6000 Gb (for a dual S4 flow cell run on the NovaSeq 6000 system)
Maximum reads per run 20 billion (for a dual S4 flow cell run on the NovaSeq 6000 system)
Nucleic acid type DNA, RNA
Reagent type Paired-End Sequencing, Sequencing Control, Cluster Generation, Sequencing by Synthesis, Individual Lane Loading, Single-Read Sequencing
Technology Sequencing

Required products

The NovaSeq 6000 Xp Workflow requires one of the following kits:

  • NovaSeq 6000 Xp 2-Lane Kit v1.5: Compatible with NovaSeq 6000 SP, S1, and S2 flow cells

  • NovaSeq 6000 Xp 4-Lane Kit v1.5: Compatible with NovaSeq 6000 S4 flow cells

These kits contain reagents and a disposable manifold for sample loading.  

 

The NovaSeq 6000 Xp Flow Cell Dock is a one-time purchase, reusable hardware accessory that is required for the NovaSeq 6000 Xp Workflow.

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Applications

Related applications and methods

Large whole-genome sequencing

Large whole-genome sequencing informs disease research and population genomics studies and reveals disease-associated alleles.

Exome sequencing

Whole-exome sequencing is used to investigate protein-coding regions of the genome to uncover genetic influences on disease and population health.

Shotgun metagenomic sequencing

Comprehensively sample all genes in all organisms present in a given complex sample to evaluate bacterial diversity and detect unculturable microorganisms.

Documentation

Product literature

Literature is not currently available for this product.

Support documentation

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Selection tools

Sequencing coverage calculator

Calculator to help determine the reagents and sequencing runs needed to arrive at desired coverage for your experiment.

Resources

NovaSeq 6000 Xp Workflow Course

v1.5 reagents (2)

NovaSeq XP 4-Lane Kit v1.5

20043131

Includes three tubes of ExAmp reagents and a single manifold for loading a four-lane NovaSeq flow cell. Used along with the NovaSeq Xp Flow Cell Dock in an optional workflow that allows accessibility to individual lanes of the NovaSeq flow cell.

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NovaSeq XP 2-Lane Kit v1.5

20043130

Includes three tubes of ExAmp reagents and a single manifold for loading a two-lane NovaSeq flow cell. Used along with the NovaSeq Xp Flow Cell Dock in an optional workflow that allows accessibility to individual lanes of the NovaSeq flow cell.

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v1 reagents (2)

NovaSeq XP 2-Lane Kit

20021664

The NovaSeq Xp 2-Lane Kit is a consumable used along with the NovaSeq Xp Flow Cell Dock in an optional workflow that allows accessibility to individual lanes of the NovaSeq flow cell. The kit consists of ExAmp reagents (3 tubes) and a single manifold needed to load a 2-lane NovaSeq flow cell.

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NovaSeq Xp 4-Lane Kit

20021665

The NovaSeq Xp 4-Lane Kit is a consumable used along with the NovaSeq Xp Flow Cell Dock in an optional workflow that allows accessibility to individual lanes of the NovaSeq flow cell. The kit consists of ExAmp reagents (3 tubes) and a single manifold needed to load a 4-lane NovaSeq flow cell.

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Accessory products (3)

NovaSeq Xp Flow Cell Dock

20021663

Includes one reusable flow cell holder for use with all NovaSeq flow cells, required for the optional NovaSeq Xp workflow.

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NovaSeq™ Xp 2-Lane Manifold Pack

20021666

Includes spare pack with 16 additional 2-lane manifolds for use with the NovaSeq Xp workflow.

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NovaSeq™ Xp 4-Lane Manifold Pack

20021667

Includes spare pack with 16 additional 4-lane manifolds for use with the NovaSeq Xp workflow.

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FAQs

The NovaSeq 6000 Xp Workflow enables sequencing of different libraries (pools) in each lane of the NovaSeq flow cell. Additionally, the ExAmp/library mix in the Xp workflow is prepared manually and loaded onto the flow cell using the NovaSeq 6000 Xp Flow Cell Dock.

The NovaSeq 6000 Xp Workflow follows the same steps as the standard workflow until after library denaturing. Denatured libraries are manually added to the ExAmp master mix and directly loaded into individual lanes of the flow cell using the NovaSeq 6000 Xp flow cell dock. The filled flow cell is then loaded onto the instrument for clustering and sequencing.

Yes. Data quality is expected to be equivalent. Please refer to this application note for details. 

The NovaSeq 6000 Xp kit is available in two sizes. The NovaSeq 6000 Xp 2-lane kit v1.5 is used with SP, S1, and S2 flow cells. The NovaSeq 6000 Xp 4-lane kit v1.5 is used with S4 flow cells. Each kit contains a single-use manifold and sufficient reagents for one NovaSeq flow cell.

The NovaSeq 6000 Xp Workflow allows for each flow cell lane to be loaded independently. This allows sequencing of different libraries in each lane of the flow cell. 
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