In May 2018, Illumina introduced the NextSeq™ 500/550 v2.5 sequencing reagent kits. With the availability of these new kits, the TruSight Cardio Sequencing Kit for NextSeq v2 will be obsolesced and upgrading to TruSight Cardio Sequencing Kit for NextSeq v2.5.
The last order date for v2 will be December 31, 2019 and the last ship date will be January 15, 2020. The library prep components of the kit have not changed. Please contact your local sales representative for additional information on this change.
|Instrument||Recommended Number of Samples||Read Length|
|MiSeq System||12 samples per run with v2 reagents (based on 300x mean coverage of targeted content)||2 × 150 bp (max recommended)|
|NextSeq 550 System||48 samples per run (mid output; based on 300x mean coverage of targeted content)||2 × 150 bp (max recommended)|
|TruSight Cardio Sequencing Kit||TruSight One Sequencing Panels||TruSight Inherited Disease|
|Content Specifications||575 Kb genomic content (174 genes)||TruSight One: ~12 Mb genomic content (~4800 genes).
TruSight One Expanded: ~16.5 Mb genomic content (~6700 genes).
|2.25 Mb genomic content (552 genes)|
|Description||Targeted sequencing research panel to identify causal variants associated with 17 inherited cardiac conditions.||Fixed research panels targeting exonic regions that harbor disease-causing variants.||Fixed research panel focused on severe pediatric onset disorders.|
|Input Quantity||50 ng DNA||50 ng DNA||50 ng DNA|
|Method||Target Enrichment , Target Enrichment, Targeted DNA Sequencing||Target Enrichment , Target Enrichment, Targeted DNA Sequencing||Target Enrichment , Target Enrichment, Targeted DNA Sequencing|
|Multiplexing||Up to 96-plex||Up to 96-plex||Up to 96-plex|
|Specialized Sample Types||Not FFPE-Compatible||Not FFPE-Compatible||Not FFPE-Compatible|
The TruSight Cardiomyopathy Sequencing Panel has been discontinued. The TruSight Cardio Sequencing Kit is the recommended replacement.
* Data on file. Illumina, Inc. 2015.