TruSight RNA Fusion Panel

Provides comprehensive gene fusion detection in formalin-fixed, paraffin-embedded (FFPE) and other cancer research samples. Read More...
Select Product(s)

TruSight RNA Fusion Panel Set A (48 samples)

RS-304-1002

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TruSight RNA Fusion Panel Set B (48 samples)

RS-304-1003

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TruSight RNA Fusion Oligo Panel

20046101

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Product Highlights

This targeted RNA sequencing panel is a cost-effective solution to detect gene fusions in multiple cancer types, regardless of origin. Covering 507 fusion-associated genes, a single assay enables researchers to assess most known cancer-related fusions in blood, bone marrow, and FFPE samples, with the power to identify novel fusion gene partners.

  • Industry-guided content for a comprehensive view of cancer-related fusion genes, detecting common and novel fusions
  • From RNA to results in 4 days including on-instrument software that displays fusion calls
  • Compatibility with desktop sequencers to maximize lab budgets
  • Optimized RNA sequencing for all sample types, including FFPE tissues
Simple Fusion Report for Customers New to NGS

On-instrument fusion calling provides NGS-based fusion detection to labs without additional bioinformatics support. The analysis provides a list of detected fusions, relevant disease associations (as identified by the Mitelman database), and evidence of fusion-supporting reads.

Robust, Reproducible, and Low-Input Fusion Assay

This assay accommodates as little input as 20 ng FFPE RNA or 10 ng fresh-frozen total RNA. The TruSight RNA Fusion Panel provides a sensitive, reproducible, and economical solution for studies of gene fusions in cancer research.

Frequently Purchased Together

Specifications

Project Recommendations

Instrument Recommended Number of Samples Read Length
MiniSeq System Samples per run: mid output: 2-3, high output: 8 (based on 3 million reads per sample) 2 × 76 bp (max recommended)
MiSeq System 8 samples per run with v3 reagents (based on 3 million reads per sample) 2 × 76 bp (max recommended)
NextSeq 550 System 24 samples per run (mid output; based on 3 million reads per sample) 2 × 76 bp (max recommended)

Product Comparison

TruSight RNA Fusion Panel TruSight Tumor 170 AmpliSeq for Illumina Focus Panel AmpliSeq for Illumina Comprehensive Panel v3
Assay Time 2.5 days ~2 days (Library Prep) 5-6 hours (library prep only; doesn't include library quantification, normalization, or pooling time) 5-6 hours (library prep only; doesn't include library quantification, normalization, or pooling time)
Cancer Type Pan-Cancer, Solid Tumor Solid Tumor Solid Tumor Solid Tumor
Hands-On Time 11 hours ~10.5 hours < 1.5 hours < 1.5 hours
Input Quantity 10 ng total RNA, 20–100 ng FFPE RNA 40 ng DNA and/or RNA 1–100 ng (10 ng recommended per pool) 1–100 ng (10 ng recommended per pool)
Method Target Enrichment, Target Enrichment, Targeted RNA Sequencing Target Enrichment, Target Enrichment, Targeted DNA Sequencing, Targeted RNA Sequencing Amplicon Sequencing, Targeted DNA Sequencing, Targeted RNA Sequencing Amplicon Sequencing, Targeted DNA Sequencing, Targeted RNA Sequencing
Nucleic Acid Type RNA RNA, DNA DNA, RNA DNA, RNA
Specialized Sample Types FFPE Tissue, Low-Input Samples FFPE Tissue, Low-Input Samples FFPE Tissue FFPE Tissue
Species Category Human Human Human Human

Method-Specific Workflow Example