A simplified, cost-effective solution for generating miRNA and other compatible small RNA libraries directly from total RNA, for any species.
Assay time
Hands-on time
Input quantity
Illumina miRNA Prep enables robust miRNA-specific library preparation while minimizing reaction biases and eliminating adapter dimers. Unique Molecular Identifiers (UMIs) tag each miRNA at an early stage, removing PCR and sequencing biases.
Illumina miRNA Prep generates reliable, miRNA-specific libraries, minimizes reaction biases, and eliminates adapter dimers, even from a low input of total RNA.
No gel electrophoresis, excision, and elution steps are required, providing a substantial reduction in sample loss and improved time savings.
Illumina miRNA Prep enables high-throughput sequencing on Illumina MiSeq, NextSeq, and NovaSeq Sequencing Systems using up to 384 unique dual indexes.
| Assay time | 7 hr |
|---|---|
| Description | Streamlined, gel-free workflow for capturing miRNA |
| Hands-on time | 3 hr |
| Input quantity | 1-500 ng RNA |
| Instruments | NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, NovaSeq X System, NovaSeq 6000 System, NovaSeq X Plus System, MiSeq i100 System |
| Method | miRNA and small RNA sequencing |
| Multiplexing | Up to 384 Unique Dual Indexes (UDIs) |
| Nucleic acid type | RNA |
| Sample type details | Supports a variety of sample types, including circulating miRNA. |
| Specialized sample types | Blood, Low-input samples, FFPE tissue |
| Species category | Drosophila, Any species, Mouse, Zebrafish, Human, Rat, Nematode |
| Strand specificity | Stranded |
| Technology | Sequencing |
| Variant class | Novel transcripts |
For 96 samples, you will need 1x Illumina miRNA Prep 96-reaction library prep kit and 1x Illumina miRNA UD Index Plate (choose A-D).
Illumina miRNA Prep enables preparation of miRNA and small RNA sequencing libraries for a wide variety of sample types in 7 hours, with 3 hours of hands-on time, using as little as 1 ng of total RNA input.
Illumina miRNA Prep (96 samples)
| Instrument | Recommended number of samples | Read length |
|---|---|---|
| NextSeq 2000 System | P2 XLEAP-SBS (100 cycles) - 96 samples P3 XLEAP-SBS (100 cycles) - 192 samples P4 XLEAP-SBS (100 cycles) - 384 samples (recommended 5-10M reads/sample) |
1 x 75 bp |
| MiSeq i100 System | 25M Reagent Kit (100 cycles) - 6 samples |
1 x 75 bp |
RNA-Seq uses next-generation sequencing to analyze expression across the transcriptome, enabling scientists to detect known or novel features and quantify RNA.
miRNA and small RNA sequencing
Isolate and sequence small RNA species, such as miRNAs and other small noncoding RNAs, or study differential expression of small RNAs.
| Illumina miRNA Prep | Illumina Stranded mRNA Prep | TruSeq Small RNA Library Preparation Kits | |
|---|---|---|---|
| Assay time | 7 hr | 6.5 hr | 1 day |
| Description | Streamlined, gel-free workflow for capturing miRNA | A simple, cost-effective solution for analysis of the coding transcriptome with precise strand information | |
| Hands-on time | 3 hr | < 3 hr | 4 hr |
| Input quantity | 1-500 ng RNA | 25-1000 ng standard-quality total RNA | |
| Instruments | NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, NovaSeq X System, NovaSeq 6000 System, NovaSeq X Plus System, MiSeq i100 System | NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, NovaSeq X System, NextSeq 500 System, NovaSeq 6000 System, NovaSeq X Plus System | MiSeq System, iSeq 100 System, NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, MiniSeq System, NextSeq 500 System |
| Method | miRNA and small RNA sequencing | mRNA sequencing | miRNA and small RNA sequencing |
| Multiplexing | Up to 384 Unique Dual Indexes (UDIs) | Up to 384 Unique Dual Indexes (UDIs) | 48 unique indexes are available |
| Nucleic acid type | RNA | RNA | RNA |
| Specialized sample types | Blood, Low-input samples, FFPE tissue | Not FFPE-compatible, Low-input samples | Low-input samples |
| Species category | Drosophila, Any species, Mouse, Zebrafish, Human, Rat, Nematode | Mammalian, Bovine, Mouse, Human, Rat | Drosophila, Any species, Mouse, Zebrafish, Human, Rat, Plant, Nematode |
| Strand specificity | Stranded | Stranded | |
| Technology | Sequencing | Sequencing | Sequencing |
Library Prep and Array Kit Selector
Find the right sequencing library preparation kit or microarray for your needs. Filter by method, species, and more. Compare, share, and order kits.
Illumina miRNA Prep chemistry
Adapters are ligated to 5' and 3' ends to ensure preferential amplification of miRNAs. Unique molecular identifiers (UMIs) are integrated during the reverse transcription (RT) step, enabling digital quantification and correcting for amplification bias. A rapid, bead-based cleanup replaces gel purification, streamlining the workflow to just seven hours.
Illumina miRNA Prep and TruSeq Small RNA workflow comparison
By eliminating gel electrophoresis and purification, Illumina miPrep mRNA Prep delivers a faster workflow with reduced hands-on time compared to TruSeq Small RNA.
Unbiased miRNA quantification using UMIs
By incorporating unique molecular identifiers (UMIs) early in the workflow, most sequencing reads are mapped to unique miRNAs, minimizing bias from adapter dimers.
Robust miRNA detection in serum samples
Illumina miRNA Prep provides robust, reproducible miRNA detection in biofluids such as serum. (A) Detection of miRNA from replicate serum samples. (B) Reads of mapped miRNAs compared to adapter dimers from replicate serum samples.
Illumina miRNA Prep (96 samples)
20145030
Includes all reagents for library prep, indexes not included
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Illumina miRNA Indexes Set A (96 Indexes, 96 Samples)
20145031
96 unique dual indexes, Set A
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Illumina miRNA Indexes Set B (96 Indexes, 96 Samples)
20145032
96 unique dual indexes, Set B
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Illumina miRNA Indexes Set C (96 Indexes, 96 Samples)
20145033
96 unique dual indexes, Set C
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Illumina miRNA Indexes Set D (96 Indexes, 96 Samples)
20145034
96 unique dual indexes, Set D
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Illumina miRNA Prep is compatible with total RNA isolated from cells, FFPE tissue, serum/plasma, and fresh/frozen tissue from virtually any species.
No miRNA enrichment is required—Illumina miRNA Prep starts with total RNA.
No, Illumina miRNA Prep is designed to work with Illumina miRNA UD Index Plates A-D and is not compatible with other Illumina RNA UDI plates.
The kit is optimized to capture small RNAs in the 18-40 base range. Other small RNAs that are compatible with Illumina miRNA Prep include PIWA-interacting RNA (piRNA), small interfering RNA (siRNA), and transfer RNA-derived small RNA (tsRNA).
Reagents for the NextSeq 1000/2000 System feature easy-to-use cartridges and multiple flow cell configurations for flexible sequencing options.
Reagents for the MiSeq i100 Series feature easy-to-use cartridges and multiple flow cell configurations for flexible sequencing options.
Streamlined RNA-Seq solution for clear and comprehensive analysis across the coding and non-coding transcriptome with exceptional study flexibility.
A rapid, cost-effective workflow for accurate, unbiased detection of the protein-coding transcriptome with precise measurement of strand information.
Simplified operations, advanced chemistry, and onboard informatics provide an easy-to-use, fast, and flexible instrument supporting a broad range of applications.
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