This video demonstrates the steps to denture and dilute prepared libraries for sequencing on the Illumina® MiSeq® system. The video will also show how to prepare and add a PhiX library for use as a sequencing control. During the video we will denature and dilute the libraries for a MiSeq V3 cartridge, following the MiSeq denature and dilute guide, Protocol A. Please check the guide at the link below for additional protocols:https://sapac.support.illumina.com/downloads/prepare_libraries_for_sequencing_miseq_15039740.html Subscribe to the Illumina video channel http://www.youtube.com/subscription_center?add_user=IlluminaInc A global genomics leader, Illumina provides comprehensive next-generation sequencing solutions to the research, clinical, and applied markets. Illumina technology is responsible for generating more than 90% of the world’s sequencing data.* Through collaborative innovation, Illumina is fueling groundbreaking advancements in oncology, reproductive health, genetic disease, microbiology, agriculture, forensic science, and beyond. MLR ID:M-GL-00702 *Data calculations on file. Illumina, Inc., 2015. View customer spotlight videos https://www.youtube.com/playlist?list=PLKRu7cmBQlajfheLzgbI4S7xBn7IDbt79 View Illumina webinars https://www.youtube.com/playlist?list=PLKRu7cmBQlahpXlnrrXlQw9itVJ8yHwUZ View Illumina product videos https://www.youtube.com/playlist?list=PLKRu7cmBQlaj6YuZmkfxZcT9twqDgP2Xd View Illumina support videos https://www.youtube.com/playlist?list=PLKRu7cmBQlajbm2KGsICWb-JOnusJfYvM 00:00 Intro 00:43 Consumables 01:39 Prepare HT1 02:22 Prepare a Fresh Dilution of NaOH 03:36 Denature a 4 nM Library 05:17 Dilute the denatured library 06:40 Dilute the PhiX control to 4 nM 07:59 Denature PhiX control 09:44 Dilute the denatured PhiX to 20 pM 10:40 Combine Library and PhiX Control