Cleaning the flow cell and flow cell stage before loading can prevent and resolve most flow cell loading issues. Flow cell loading issues include vacuum issues, flow cell RFID issues and consumable loading issues. This video describes best practices on how to prevent and resolve flow cell loading issues on the NovaSeq 6000. QB 10550 Resources: What should I do if the NovaSeq™ 6000 flow cell cannot be loaded during run setup? https://support.illumina.com/bulletins/2020/06/what-should-i-do-if-the-novaseq--6000-flow-cell-cannot-be-loaded.html NovaSeq™ 6000 Power Cycle https://www.youtube.com/watch?v=I3KP51ID56I&feature=youtu.be Subscribe to the Illumina video channel http://www.youtube.com/subscription_center?add_user=IlluminaInc A global genomics leader, Illumina provides comprehensive next-generation sequencing solutions to the research, clinical, and applied markets. Illumina technology is responsible for generating more than 90% of the world’s sequencing data.* Through collaborative innovation, Illumina is fueling groundbreaking advancements in oncology, reproductive health, genetic disease, microbiology, agriculture, forensic science, and beyond. *Data calculations on file. Illumina, Inc., 2015. View customer spotlight videos https://www.youtube.com/playlist?list=PLKRu7cmBQlajfheLzgbI4S7xBn7IDbt79 View Illumina webinars https://www.youtube.com/playlist?list=PLKRu7cmBQlahpXlnrrXlQw9itVJ8yHwUZ View Illumina product videos https://www.youtube.com/playlist?list=PLKRu7cmBQlaj6YuZmkfxZcT9twqDgP2Xd View Illumina support videos https://www.youtube.com/playlist?list=PLKRu7cmBQlajbm2KGsICWb-JOnusJfYvM