Getting to know your NovaSeq™ X Plus

8 August 2023

The NovaSeq™ X series offers the throughput and accuracy needed to enable more data-intensive applications and deliver meaningful insights at scale. Watch and learn from Illumina Training & Development Specialist, Ching-Aeng Lim, PhD how to get started with your NovaSeq X. Planning your run, preparing your libraries and consumables for sequencing, and setting up the instrument. Find out how you can achieve outstanding throughput and scalability without sacrificing flexible, streamlined, and easy-to-use workflows. https://www.illumina.com/systems/sequencing-platforms/novaseq-x-plus.html Chaptering: 0:00 Getting started with your NovaSeq X Plus 0:31 1. Planning your run – creating a run, run settings, FASTQ file compression format, DRAGEN™ software, cycles, analysis configuration, pipeline. 1:34 • Selecting your library prep – library prep, reference genome 1:54 • Desired variant callers – entering a list of samples, generating library pool, importing information in bulk, sample list template. 3:01 • Map/Align Output Format – CRAM, FASTQ files, run review, multiple analysis configurations on a single flow cell. 3:54 2. Library Prep & Consumables – receiving reagents and consumables, shipments at ambient temperature, step-by-step library prep. 5:02 3. Setting up your run – flow cell, dual flow cell run, run parameters, loading consumables 5:30 • Loading flow cells – 10B flow cell, cluster density, open and close flow cell door. 6:09 • Loading reagents and buffers – open and close doors, drawers, buffer cartridge, wash cartridge, waste drawers, waste bottle, verify run and consumables. 7:00 • Verify your run – pre-run checks cluster generation and sequencing start, on-board DRAGEN analysis kickstart 7:20 4. Monitoring the progress of your run – use BaseSpace™ Sequencing Hub to monitor the progress of your run from your device, from anywhere. Subscribe to the Illumina video channel http://www.youtube.com/subscription_center?add_user=IlluminaInc A global genomics leader, Illumina provides comprehensive next-generation sequencing solutions to the research, clinical, and applied markets. View customer spotlight videos https://www.youtube.com/playlist?list=PLKRu7cmBQlajfheLzgbI4S7xBn7IDbt79 View Illumina webinars https://www.youtube.com/playlist?list=PLKRu7cmBQlahpXlnrrXlQw9itVJ8yHwUZ View Illumina product videos https://www.youtube.com/playlist?list=PLKRu7cmBQlaj6YuZmkfxZcT9twqDgP2Xd View Illumina support videos https://www.youtube.com/playlist?list=PLKRu7cmBQlajbm2KGsICWb-JOnusJfYvM Facebook: https://www.facebook.com/illuminainc LinkedIn: https://www.linkedin.com/company/illu... Instagram: https://www.instagram.com/illuminainc/ Twitter: https://twitter.com/illumina

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