Sequence thousands of organisms in parallel

Comprehensively sample all genes in all organisms present in a complex sample

Shotgun Metagenomic Sequencing

Shotgun metagenomic sequencing allows researchers to comprehensively sample all genes in all organisms present in a given complex sample. The method enables microbiologists to evaluate bacterial diversity and detect the abundance of microbes in various environments. Shotgun metagenomics also provides a means to study unculturable microorganisms that are otherwise difficult or impossible to analyze.

Unlike capillary sequencing or PCR-based approaches, next-generation sequencing (NGS) allows researchers to sequence thousands of organisms in parallel. With the ability to combine many samples in a single sequencing run and obtain high sequence coverage per sample, NGS-based metagenomic sequencing can detect very low abundance members of the microbial community that may be missed or are too expensive to identify using other methods.

16S rRNA Sequencing is another method used for metagenomics studies.

There are multiple ways to perform shotgun-based metagenomic sequencing experiments, but these are some suggested products for each step of the workflow.

Click on the below to view products for each workflow step.

Nextera DNA Flex Library Prep Kit

A fast, integrated workflow for a wide range of applications, from human whole-genome sequencing to amplicons, plasmids, and microbial species.

Nextera XT Library Prep Kit

Prepare sequencing-ready libraries for small genomes (bacteria, archaea, viruses), amplicons, and plasmids in less than 90 minutes.

TruSeq DNA PCR-Free Library Preparation Kits

Simple, all-inclusive library preparation for whole-genome sequencing applications. Researchers can sequence a wide variety of organisms, from small genomes such as bacteria to whole-human genomes.

Benchtop Sequencing

MiSeq System

Speed, accuracy and simplicity for far-reaching applications in microbiology.

NextSeq 550 System

Flexible desktop sequencer for exome, transcriptome, and whole-genome sequencing.

High-Throughput Sequencing
Power for shotgun metagenomics and flexibility to scale based on your project or workflow needs.

NovaSeq 6000 System

High throughput, low cost for production-scale genomics.

BaseSpace Apps for k-mer alignments and taxonomic classification

Kraken Metagenomics

Assigns taxonomic labels to short DNA sequences with high sensitivity and speed using exact alignments of k-mers and a novel classification algorithm.

One Codex

Enables metagenomic analysis by rapidly assessing your samples from BaseSpace Sequence Hub with a fast, comprehensive, accurate data platform

GENIUS Metagenomics: Know Now

Uses CosmosID's curated genome database and high performance algorithms to provide rapid, accurate, and actionable bacterial identification at the species, subspecies, and strain level.

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