RNA sequencing (RNA-Seq) is revolutionizing the study of the transcriptome. A highly sensitive and accurate tool for measuring expression across the transcriptome, it is providing researchers with visibility into previously undetected changes occurring in disease states, in response to therapeutics, under different environmental conditions, and across a broad range of other study designs.
RNA-Seq allows researchers to detect both known and novel features in a single assay, enabling the detection of transcript isoforms, gene fusions, single nucleotide variants, and other features without the limitation of prior knowledge.
RNA-Seq with next-generation sequencing (NGS) is increasingly the method of choice for researchers studying the transcriptome. It offers numerous advantages over gene expression arrays.
Find out how Illumina NGS technology works and what types of experiments it enables.About NGS Technology
Learn about 7 key RNA-Seq methods. Find out how they differ to help you determine the method most appropriate for your research.Show Key RNA-Seq Methods
Benchtop sequencer supporting multiple applications, including up to 16 mRNA samples in a single run.
RNA-Seq is by far the most cited NGS method. This collection contains protocol diagrams, advantages and disadvantages, and related peer-reviewed publications on various RNA-Seq methods featuring Illumina technology.Read Review