Study gene expression using RNA sequencing

A high-resolution view of coding and noncoding regions of the transcriptome for a deeper understanding of biology

RNA Sequencing

RNA sequencing (RNA-Seq) is revolutionizing the study of the transcriptome. A highly sensitive and accurate tool for measuring expression across the transcriptome, it is providing researchers with visibility into previously undetected changes occurring in disease states, in response to therapeutics, under different environmental conditions, and across a broad range of other study designs.

RNA-Seq allows researchers to detect both known and novel features in a single assay, enabling the detection of transcript isoforms, gene fusions, single nucleotide variants, and other features without the limitation of prior knowledge.

RNA-Seq with next-generation sequencing (NGS) is increasingly the method of choice for researchers studying the transcriptome. It offers numerous advantages over gene expression arrays.

  • Broader dynamic range enables more sensitive and accurate measurement of gene expression
  • Not limited by prior knowledge - captures both known and novel features
  • Can be applied to any species, even if reference sequencing is not available
  • A better value, often delivering advantages at a comparable or lower price per sample than many arrays
Propelling Progress with RNA-Seq
Propelling Progress with RNA-Seq

RNA sequencing can have far-reaching effects on research and innovation, transforming our understanding of the world around us.

RNA Sequencing Empowers Transcriptomics

Learn how RNA-Seq is advancing transcriptome research in various fields, and how gene regulation studies can provide complementary information.

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New To NGS?

Find out how Illumina NGS technology works and what types of experiments it enables.

About NGS Technology 
RNA-Seq vs. Microarrays 
RNA-Seq vs. qRT-PCR 
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How Can I Use NGS to Analyze RNA?

Learn about 7 key RNA-Seq methods. Find out how they differ to help you determine the method most appropriate for your research.

Show Key RNA-Seq Methods 
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How Can I Apply RNA-Seq?

Study gene expression and transcriptome changes with cancer RNA-Seq.

Analyze host-pathogen interactions or bacterial transcriptome signatures with microbial RNA-Seq.

Study drug response RNA biomarkers.

RNA Sequencing Publication Review

RNA-Seq is by far the most cited NGS method. This collection contains protocol diagrams, advantages and disadvantages, and related peer-reviewed publications on various RNA-Seq methods featuring Illumina technology.

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RNA Sequencing Publication Review

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Deeper Insights with RNA-Seq
Deeper Insights with RNA-Seq

RNA sequencing provides deeper insights for complex research. See how RNA-Seq is helping this lab move beyond gene expression.

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RNA Sequencing Considerations
RNA Sequencing Considerations

Learn about read length and depth requirements for RNA-Seq and find resources to help with experimental design.

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FAQs: RNA-Seq Data Analysis
FAQs: RNA-Seq Data Analysis

Demystifying data analysis with answers to some of the most common frequently asked questions.

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Buyer’s Guide: Simple RNA-Seq Workflows
Buyer’s Guide: Simple RNA-Seq Workflows

Evaluating options for next-generation RNA sequencing.

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Methods Guide
Methods Guide

All the information you need, from BeadChips to library preparation to sequencer selection and analysis. Select the best tools for your lab.

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