This is part three of a five-part series about the Illumina Single Cell 3' RNA Prep. Part three covers Supplemental Enrichment and Amplification (SEA) Kit applications and primer design. Subscribe to the Illumina video channel http://www.youtube.com/subscription_center?add_user=IlluminaInc A global genomics leader, Illumina provides comprehensive next-generation sequencing solutions to the research, clinical, and applied markets. Through collaborative innovation, Illumina is fueling groundbreaking advancements in oncology, reproductive health, genetic disease, microbiology, agriculture, forensic science, and beyond. View customer spotlight videos https://www.youtube.com/playlist?list=PLKRu7cmBQlajfheLzgbI4S7xBn7IDbt79 View Illumina webinars https://www.youtube.com/playlist?list=PLKRu7cmBQlahpXlnrrXlQw9itVJ8yHwUZ View Illumina support videos https://www.youtube.com/playlist?list=PLKRu7cmBQlajbm2KGsICWb-JOnusJfYvM LinkedIn: https://www.linkedin.com/company/illumina X: https://x.com/illumina Instagram: https://www.instagram.com/illuminainc/ Facebook: https://www.facebook.com/illuminainc TikTok: https://www.tiktok.com/@illuminainc #IlluminaSingleCellRNAPrep #SingleCell #illumina 00:00 - Introduction 00:11 - Experimental Design Recommendations 01:35 - User Guides 02:03 - Supplemental Enrichment and Amplification (SEA) Kit Applications 06:56 - Reply Application 6:56 09:13 - Synthetic Nucleotide Tag (SNT) Application 14:14 - CROPSeq gRNA Capture Application 15:35 - Targeted Amplicon Application 18:06 - Experiment Design Considerations