This is part two of a five-part series about the Illumina Single Cell 3’ RNA prep. Part two covers more sample prep best practices including nuclei preparations and enrichment by FACS and MACS. Subscribe to the Illumina video channel http://www.youtube.com/subscription_center?add_user=IlluminaInc A global genomics leader, Illumina provides comprehensive next-generation sequencing solutions to the research, clinical, and applied markets. Through collaborative innovation, Illumina is fueling groundbreaking advancements in oncology, reproductive health, genetic disease, microbiology, agriculture, forensic science, and beyond. View customer spotlight videos https://www.youtube.com/playlist?list=PLKRu7cmBQlajfheLzgbI4S7xBn7IDbt79 View Illumina webinars https://www.youtube.com/playlist?list=PLKRu7cmBQlahpXlnrrXlQw9itVJ8yHwUZ View Illumina support videos https://www.youtube.com/playlist?list=PLKRu7cmBQlajbm2KGsICWb-JOnusJfYvM LinkedIn: https://www.linkedin.com/company/illumina X: https://x.com/illumina Instagram: https://www.instagram.com/illuminainc/ Facebook: https://www.facebook.com/illuminainc TikTok: https://www.tiktok.com/@illuminainc #IlluminaSingleCellRNAPrep #RNASingleCell 00:00 - Introduction 00:10 - Experimental Design: Recommendations 01:59 - Sample Preparation: Cell or Nuclei 04:44 - Sample Preparation: Dead Cell Removal Kits 06:06 - Sample Preparation: Nuclei 10:35 - Sample Preparation: Enrichment strategies 12:10 - Sample Preparation: Flow Sorting Recommendations