How to Leverage Illumina Sequencing in Genome-Wide CRISPR Screens

14 December 2021

Learn about how Clustered Regularly Interspaced Short Palindromic repeats (CRISPR) screens can be an important tool in your research, as well as how to perform a successful experiment using NGS as a downstream readout. Presenter: Lauren Fait– Sr. Sequencing Specialist, Illumina 00:00 Introduction 00:32 CRISPR Screening Overview 2:28 Natural CRISPR Systems Have 2 Major Components 5:11 CRISPR Screen Overall Workflow 6:49 sgRNA Library Assembly and Packaging 9:04 NGS Quality Control for Pooled Libraries 10:05 Pooled CRISPR Screen 15:12 Genome-Scale Knockout Libraries 18:00 Hit Identification using Illumina Sequencing 20:58 Single-Cell Sequencing for CRISPR Screens Subscribe to the Illumina video channel http://www.youtube.com/subscription_center?add_user=IlluminaInc A global genomics leader, Illumina provides comprehensive next-generation sequencing solutions to the research, clinical, and applied markets. Illumina technology is responsible for generating more than 90% of the world’s sequencing data.* Through collaborative innovation, Illumina is fueling groundbreaking advancements in oncology, reproductive health, genetic disease, microbiology, agriculture, forensic science, and beyond. *Data calculations on file. Illumina, Inc., 2015. View customer spotlight videos https://www.youtube.com/playlist?list=PLKRu7cmBQlajfheLzgbI4S7xBn7IDbt79 View Illumina webinars https://www.youtube.com/playlist?list=PLKRu7cmBQlahpXlnrrXlQw9itVJ8yHwUZ View Illumina product videos https://www.youtube.com/playlist?list=PLKRu7cmBQlaj6YuZmkfxZcT9twqDgP2Xd View Illumina support videos https://www.youtube.com/playlist?list=PLKRu7cmBQlajbm2KGsICWb-JOnusJfYvM

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