Nextera DNA Exome

Nextera DNA Exome

The most efficient Exome workflow focused on 45 Mb of coding sequence from >98% of RefSeq, CCDS, and Ensembl coding content.

Nextera DNA Exome Pre-Assembled kits
Catalog Number Total Samples Number of Enrichments Indexing
20020616 24 8 24 combinatorial dual indexes
20020617 96 8 96 combinatorial dual indexes

Nextera DNA Exome — Choose Your Components*
Library Preparation for Enrichment
Catalog Number Total Samples
20020187 24
20020188 96
Index Primers
Catalog Number Total Samples Indexing
20018707 24 24 combinatorial dual indexes
20018708 96 96 combinatorial dual indexes
* When choosing your own components, Panel and Enrichment Reagents can be purchased through IDT

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Project Recommendations*
Species Compatibility Human
Recommended Illumina Instrument NextSeq 500 HiSeq 2500 (Dual Flow Cell) NovaSeq 6000 (Dual Flow Cell)†
Recommended Samples per run‡ 3 12 24 156 96 96 192
Run Mode Mid Output High Output Rapid Run High Output S1§ S2 S4
Max Recommended Read Length 2×75 bp 2×75 bp 2×75 bp 2×75 bp ≤ 2×100 bp
Recommended Secondary Analysis
  • BWA Enrichment BaseSpace App
  • Isaac Enrichment v2 BaseSpace App
Recommended Down Stream Analysis VariantStudio
* Library prep is compatible with all Illumina instruments, except the X5 and X10 systems. Recommended instruments chosen based on typical output and analysis need for selected application.
† Limited by index combinations (HT).
‡ 50M Reads, 50× Coverage, 4GB of Data.
§ NovaSeq S1 Reagent Kits are not currently available.
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For Research Use Only

Not for use in diagnostic procedures (except as specifically noted).

Innovative technologies

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