Nextera Mate Pair

Nextera Mate Pair

The only gel free method for preparing up to 12 kb mate pair libraries with a low DNA input requirement. It is an ideal approach for de novo sequencing, genome finishing, and the detection of structural variation in complex genomes.

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* Library prep is compatible with all Illumina instruments. Recommended instruments chosen based on typical output and analysis need for selected application. 
† Based on finishing of eukaryotic genome.

Project Recommendations
Species Compatibility Compatible with most large DNA genomes
Recommended Illumina Instrument* NextSeq500 HiSeq 2500 (Dual Flow Cell)
Recommended Samples per run 1 10-15
Run Mode Mid Output High Output Rapid Run High Output
Max Recommended Read Length Up to 2x150 bp Up to 2x150 bp Up to 2x150 bp Up to 2x150 bp
Recommended Secondary Analysis
  • Velvet de novo Assembly BaseSpace App
  • SPAdes Genome Assembler BaseSpace App