Nextera Rapid Capture Exome – Targeted WES

Nextera Rapid Capture Exome – Targeted WES

The most efficient Exome workflow focused on 37 Mb of coding sequence from > 98% of RefSeq, CCDS, and Ensembl coding content.

Catalog ID(s): # of Index Combinations Pre-Enrichment Multiplexing Enrichment Rxns Per Kit Maximum Samples Per Kit
FC-104-1000 1 1 8 8
FC-100-1001 24 1-12 2 24
FC-100-1002 24 1-12 4 48
FC-100-1003 24 1-12 8 96
FC-140-1083 3 1-3 8 24
FC-140-1086 6 1-6 8 48
FC-140-1089 9 1-9 8 96

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* Library prep is compatible with all Illumina instruments. Recommended instruments chosen based on typical output and analysis need for selected application.
 Based on 4 Gb per sample.

Project Recommendations
Species Compatibility Human
Recommended Illumina Instrument* MiniSeq NextSeq 500 HiSeq 2500 (Dual Flow Cell)
Recommended Samples per run Use the Nextera Rapid Capture Calculator Up to 9 Up to 150
Run Mode Mid Output High Output Mid Run High Output Rapid Run High Output
Max Recommended Read Length Up to 2x150 bp Up to 2x150 bp Up to 2x150 bp Up to 2x150 bp Up to 2x150 bp Up to 2x125 bp
Recommended Secondary Analysis
  • Local Run Manager Enrichment
  • BaseSpace BWA Enrchiment
  • BaseSpace Isaac Enrichment
  • BWA Enrichment BaseSpace App
  • Isaac Enrichment v2 BaseSpace App
Recommended Down Stream Analysis VariantStudio