Nextera Rapid Capture Expanded Exome

Nextera Rapid Capture Expanded Exome

The most comprehensive Exome workflow with an expanded focus on coding, UTR, and micro RNA regions. This product targets 62 Mb of sequence and utilizes the same enrichment oligos used in the previous TruSeq and Nextera Exome Enrichment Kits.

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Catalog ID(s): # of Index Combinations Pre-Enrichment Multiplexing Enrichment Rxns Per Kit Maximum Samples Per Kit
FC-104-1004 24 1-12 2 24
FC-140-1005 24 1-12 4 48
FC-140-1006 24 1-12 8 96

* Library prep is compatible with all Illumina instruments. Recommended instruments chosen based on typical output and analysis need for selected application.
Based on 6 Gb per sample.

Project Recommendations
Species Compatibility Human
Recommended Illumina Instrument* NextSeq 500 HiSeq 2500 (Dual Flow Cell)
Recommended Samples per run Up to 6 Up to 64
Run Mode Mid Run High Output Rapid Run High Output
Max Recommended Read Length Up to 2x150 bp Up to 2x150 bp Up to 2x150 bp Up to 2x125 bp
Recommended Secondary Analysis
  • BWA Enrichment BaseSpace App
  • Isaac Enrichment v2 BaseSpace App
Recommended Down Stream Analysis VariantStudio