Nextera XT DNA

Nextera XT DNA

The Nextera XT DNA Library Preparation Kit allows the preparation of sequencing-ready libraries for small genomes (bacteria, archaea, viruses) in less than 90 minutes with only 15 minutes of hands-on time.

Catalog ID(s):
FC-131-1024 Nextera XT DNA Library Prep Kit (24 samples)
FC-131-1096 Nextera XT DNA Library Prep Kit (96 samples)

Even if you do not plan to index, use one of the following Nextera XT Index kits (note that these index kits are not chemically equivalent to the Nextera DNA index kits [not interchangeable])

FC-131-1001 Nextera XT Index Kit (24 indices, 96 samples)
FC-131-1002 Nextera XT Index Kit (96 indices, 384 samples)
FC-131-2001 Nextera XT Index Kit v2 Set A (96 indices, 384 samples)
FC-131-2002 Nextera XT Index Kit v2 Set B (96 indices, 384 samples)
FC-131-2003 Nextera XT Index Kit v2 Set C (96 indices, 384 samples)
FC-131-2004 Nextera XT Index Kit v2 Set D (96 indices, 384 samples)

* These index kits are not interchangeable with the Nextera DNA index kits.

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* Library prep is compatible with all Illumina instruments. Recommended instruments chosen based on typical output and analysis need for selected application.
† Based on 30x coverage of a 5 Mb genome.
Project Recommendations
Species Compatibility Compatible with most small DNA genomes ≤ 20 Mb
Recommended Illumina Instrument* MiSeq NextSeq
Recommended Samples per run† Up to 100 Up to 384
Run Mode - Mid Output High Output
Max Recommended Read Length Up to 2x300 bp Up to 2x150 bp Up to 2x150 bp
Recommended Secondary Analysis
  • MiSeq Reporter Resequencing
  • IMiSeq Reporter Assembly
  • BWA WGS BaseSpace App
  • Isaac WGS v2 BaseSpace App
  • SPAdes Genome Assembler
Recommended Down Stream Analysis VariantStudio VariantStudio
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For Research Use Only

Not for use in diagnostic procedures (except as specifically noted).

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