Clontech™ SMARTer Ultra Low Input RNA can be used to process samples containing as little as 10 pg of total RNA. The workflow involves synthesizing RNA strands into cDNA and processing the cDNA into Illumina compatible libraries.
The Nextera XT kit is optimal for preparing an Illumina library from cDNA generated with Clontech™ SMARTer Ultra low input RNA kit. Simply take 1 ng of cDNA as input into the standard Nextera XT workflow. Then follow the Nextera XT protocol as written-there is no need to dilute or modify the Nextera XT enzymes or reagents.