SureCell WTA 3′ Library Prep Kit
Illumina Bio-Rad SureCell WTA 3′ Library, for ddSEQ SCI
SureCell WTA 3′ Library Prep kit provides a validated process for single-cell RNA sequencing allowing for the quick isolation and barcoding of ~1,200 cells per cartridge from multiple sources using the Bio-Rad ddSEQ Single-Cell Isolator. The kit also provides quick and easy library preparation using Illumina's Nextera XT chemistry. This kit provides cartridges, all necessary reagents for library preparation, and required custom sequencing primer.
- Starting Material
- 45,000 cells per cartridge (4 samples per cartridge).
- 11,250 cells are used as input for each sample. A library created from one sample yields sequencing data from at least 300 ce lls.
- Catalog ID(s):
- 20014279 SureCell WTA 3′ Library Prep Kit (2 cartridge kit), 8 samples
- 20014280 SureCell WTA 3′ Library Prep Kit (6 cartridge kit), 24 samples
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| Species Compatibility | Human, mouse, rat, Drosophila, C. elegans, zebra fish | ||
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| Recommended Illumina Instrument | NextSeq | HiSeq 2500 | |
| Recommended Samples per run* | 4-6 | 4–5 per flow cell | 4 per lane |
| Run Mode | High Output | Rapid Run | High Output V4 |
| Recommended Read Length | Read 1: 68 bp Read 2: 75 bp |
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| Recommended Secondary Analysis | SureCell RNA Single-Cell BaseSpace App | ||
| Recommended Down Stream Analysis | SureCell RNA Single-Cell BaseSpace App SeqGeq by FlowJo |
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* based on a minimum of 100,000 reads per cell