TruSeq Amplicon Cancer Panel

TruSeq Amplicon Cancer Panel

The TruSeq Amplicon Cancer Panel (TSACP) product includes an Illumina validated oligo pool that generates 212 amplicons targeting mutational hotspots from 48 genes associated with both solid and hematological cancers.

Catalog ID(s)
FC-130-1008 (96 samples)
FC-130-1003 (96 indices, 384 samples)

For FFPE samples, we recommend using the Illumina FFPE QC kit (WG-321-1001) to prequalify your samples. The FFPE Restore Kit is not compatible with sequencing.

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TruSeq Amplicon Cancer Panel Gene List

ABL1 AKT1
CTNNB1 ALK
EGFR APC
ERBB2 ATM BRAF CDH1 CDKN2A CSF1R ERBB4 FBXW7
HNF1A FGFR1
HRAS FGFR2
IDH1 FGFR3 FLT3 GNA11 GNAQ GNAS JAK2 JAK3
NPM1 KDR
NRAS KIT
PDGFRA KRAS MET MLH1 MPL NOTCH1 PIK3CA PTEN
STK11 PTPN11
TP53 RB1
VHL RET SMAD4 SMARCB1 SMO SRC

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* Library prep is compatible with all Illumina instruments. Recommended instruments chosen based on typical output and analysis need for selected application.
† Based on ~1,000x average coverage.
Project Recommendations
Species Compatibility Human
Recommended Illumina Instrument* MiniSeq MiSeq NextSeq 500
Recommended Samples per run† 12 40 24 (v2 chemistry) 96 96
Run Mode Mid Output High Output - Mid Output High Output
Max Recommended Read Length Up to 2x150 bp Up to 2x150 bp Up to 2x150 bp Up to 2x150 bp Up to 2x150 bp
Recommended Secondary Analysis
  • Local Run Manager Amplicon
  • BaseSpace TruSeq Amplicon
  • MiSeq Reporter TruSeq Amplicon Workflow
  • TruSeq Amplicon v1.1 BaseSpace App
Recommended Down Stream Analysis VariantStudio
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For Research Use Only

Not for use in diagnostic procedures (except as specifically noted).

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