Chromatin immunoprecipitation (ChIP) sequencing leverages next-generation sequencing (NGS) to quickly and efficiently determine the distribution and abundance of DNA-bound targets of interest across the genome. Illumina TruSeq ChIP Sample Preparation Kits require 5–10 ng of DNA and provides 24 indices.
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| * Library prep is compatible with all Illumina instruments. Recommended instruments chosen based on typical output and analysis need for selected application. † Coverage based on 15M reads per sample for identification of transcription factor binding. Other applications require different coverage |
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| Project Recommendations | ||||
|---|---|---|---|---|
| Species Compatibility | Compatible with any species | |||
| Recommended Illumina Instrument* | NextSeq 500 | HiSeq 2500 (Dual Flow Cell) | ||
| Recommended Samples per run | 8–24 | 40–264 | ||
| Run Mode | Mid Output | High Output | Rapid Run | High Output |
| Recommended Read Length | 1x50 bp | 1x50 bp | 1x50 bp | 1x50 bp |
| Recommended Secondary Analysis |
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