TruSeq Custom Amplicon v1.5

TruSeq Custom Amplicon v1.5

TruSeq Custom Amplicon (TSCA) is a fully customizable multiplexed amplicon assay for the MiSeq, with up to 1536 targets. If you are working with FFPE samples, we recommend designing your amplicons to be either 150 bp or 175 bp because FFPE samples tend to be more heavily fragmented than high-quality DNA.

To generate a custom amplicon panel:
Go to the TruSeq Custom Amplicon Custom Products webpage, select the DesignStudio link.
Begin to design the probes for the desired targeted regions.
Order the design. All designs include the probes and reagents for 96 reactions.
Order an index kit part number FC-130-1003 (96 indices, 384 samples). This kit is required when using the TruSeq Custom Amplicon option.
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Project Recommendations
Species Compatibility Human, Mouse, Rat, Bovine
Recommended Illumina Instrument* MiniSeq MiSeq NextSeq 500
Run Mode Mid Output High Output – Mid Output High Output
Max Recommended Read Length Up to 2×150 bp Up to 2×150 bp Up to 2×300 bp Up to 2×150 bp Up to 2×150 bp
Recommended Secondary Analysis
  • Local Run Manager Amplicon or Amplicon DS
  • BaseSpace TruSeq Amplicon
  • BaseSpace Amplicon DS
  • MiSeq Reporter TruSeq Amplicon Workflow
  • TruSeq Amplicon v1.1 BaseSpace App
Recommended Down Stream Analysis VariantStudio

*Library prep is compatible with all Illumina instruments. Recommended instruments chosen based on typical output and analysis need for selected application.

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For Research Use Only

Not for use in diagnostic procedures (except as specifically noted).

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