TruSeq DNA PCR-Free
TruSeq DNA PCR-Free
LT HT
Simple, all-inclusive prep solution, ideal for whole-genome sequencing. This new workflow, which accelerates the most widely adopted library prep chemistry, removes the need for PCR, enables improved genome coverage and the ability to sequence through challenging regions. The LT option is available in Set A and Set B. Each set contains 12 unique single indexes that can be pooled together for up to 24plex.
Simple, all-inclusive prep solution, ideal for whole-genome sequencing. This new workflow, which accelerates the most widely adopted library prep chemistry, removes the need for PCR, enables improved genome coverage and the ability to sequence through challenging regions. The HT option provides dual index combinations for up to 96plex.
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| Species Compatibility | Compatible with most large DNA genomes | ||||||
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| Recommended Illumina Instrument | NextSeq 500 | HiSeq 2500 (Dual Flow Cell) | NovaSeq 6000 (Dual Flow Cell) | ||||
| Recommended Samples per run† | 1 | 2–10 | 8 | 16 | 48 | ||
| Run Mode | Mid Output | High Output | Rapid Run | High Output | S1‡ | S2 | S4 |
| Max Recommended Read Length | 350 bp insert: ≤ 2×100 bp 550 bp insert: ≤ 2×150 bp |
350 bp insert: ≤ 2×100 bp 550 bp insert: ≤ 2×150 bp |
350 bp insert: ≤ 2×100 bp 550 bp insert: ≤ 2×250 bp |
350 bp insert: ≤ 2×100 bp 550 bp insert: ≤ 2×125 bp |
350 bp insert: ≤ 2×150 bp 550 bp insert: ≤ 2×150 bp |
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| Recommended Secondary Analysis |
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| Recommended Secondary Analysis |
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* Library prep is compatible with all Illumina instruments. Recommended instruments chosen based on typical output and analysis need for selected application.
† Based on 30× coverage of a human genome.
‡ NovaSeq S1 Reagent Kits are not currently available.