TruSeq RNA Access — Low Input
TruSeq RNA Access — Low Input
TruSeq RNA Access Library Prep kits convert total RNA into template molecules of known strand origin, followed by the targeted capture of coding RNA via sequence-specific capture probes, enabling cost-efficient, multi-plexed sequencing in both high and low-quality / FFPE samples. Starting material: Compatible with a wide range of RNA input quality. Illumina recommends using the percentage of RNA fragments > 200 nt distribution value (or DV200) as an indicator of RNA quality to ensure proper performance.
| Catalog ID(s): | Indexes | Pre-Enrichment Multiplexing | Enrichment | Maximum Samples Per Kit |
|---|---|---|---|---|
| RS-301-2001 | Set A: 12 indices | 1-4 | 12/kit | 48 |
| RS-301-2002 | Set B: 12 indices | 1-4 | 12/kit | 48 |
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| * Library prep is compatible with all Illumina instruments. Recommended instruments chosen based on typical output and analysis need for selected application. † Based on 25M reads per sample. |
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| Project Recommendations | ||||
|---|---|---|---|---|
| Species Compatibility | Human | |||
| Recommended Illumina Instrument* | NextSeq 500 | HiSeq 2500 (Dual Flow Cell) | ||
| Recommended Samples per run† | 5-16 | 24–160 | ||
| Run Mode | Mid Output | High Output | Rapid Run | High Output |
| Max Recommended Read Length | 2x75 bp | 2x75 bp | 2x75 bp | 2x75 bp |
| Recommended Secondary Analysis |
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| Recommended Down Stream Analysis |
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