TruSeq RNA Access
TruSeq RNA Access
TruSeq RNA Access Library Prep kits convert total RNA into template molecules of known strand origin, followed by the targeted capture of coding RNA via sequence-specific capture probes, enabling cost-efficient, multi-plexed sequencing in both high and low-quality / FFPE samples.
Starting material: Compatible with a wide range of RNA input quality. Illumina recommends using the percentage of RNA fragments >200 nt distribution value (or DV200) as an indicator of RNA quality to ensure proper performance.
| Catalog ID(s): | Indexes | Pre-Enrichment Multiplexing | Enrichment | Maximum Samples Per Kit |
|---|---|---|---|---|
| RS-301-2002 | Set A: 12 indices | 1-4 | 12/kit | 48 |
| RS-301-2001 | Set B: 12 indices | 1-4 | 12/kit | 48 |
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| Species Compatibility | Human | ||||||
|---|---|---|---|---|---|---|---|
| Recommended Illumina Instrument | NextSeq 500 | HiSeq 2500 (Dual Flow Cell) | NovaSeq 6000 (Dual Flow Cell)† | ||||
| Recommended Samples per run‡ | 5–16 | 24–160 | 96 | 96 | 192 | ||
| Run Mode | Mid Output | High Output | Rapid Run | High Output | S1§ | S2 | S4 |
| Max Recommended Read Length | 2×75 bp | 2×75 bp | 2×75 bp | 2×75 bp | 2×100 bp | ||
| Recommended Secondary Analysis |
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| Recommended Down Stream Analysis |
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* Library prep is compatible with all Illumina instruments. Recommended instruments chosen based on typical output and analysis need for selected application.
† Currently limited by available index combinations.
‡ Based on 25M reads per sample.
§ NovaSeq S1 Reagent Kits are not currently available.