TruSeq RNA v2
TruSeq RNA v2
TruSeq RNA v2 provides a simple, cost-effective solution for generating poly-A mRNA libraries from total RNA using oligo dT beads.
Starting material: Optimized for 100 ng total RNA, up to 1ug total RNA can be used, or entire fraction of mRNA purified from 0.1-1ug total RNA.
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| * Library prep is compatible with all Illumina instruments. Recommended instruments chosen based on typical output and analysis need for selected application. † Based on 10M reads per sample for RNA profiling and 25M reads per sample for transcriptome analysis. |
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| Project Recommendations | ||||
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| Species Compatibility | Any high-quality RNA species with polyA tail | |||
| Recommended Illumina Instrument* | NextSeq 500 | HiSeq 2500 (Dual Flow Cell) | ||
| Recommended Samples per run† | RNA Profiling: 13–40 Transcriptome Analysis: 5–16 |
RNA Profiling: 60–400 Transcriptome Analysis: 24–160 |
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| Run Mode | Mid Output | High Output | Rapid Run | High Output |
| Max Recommended Read Length | 2x75 bp | 2x75 bp | 2x75 bp | 2x75 bp |
| Recommended Secondary Analysis |
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| Recommended Down Stream Analysis |
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