TruSeq Stranded mRNA + Ribo-Zero

TruSeq Stranded mRNA + Ribo-Zero

In order to prepare RNA samples for whole-transcriptome analysis from a source that is not animal, plant, or bacteria, use the Epicentre's MatchMaker Tool™ to test Ribo-Zero compatibility in silico with your species. Note that the analysis is done in silico and does not guarantee rRNA removal. Ribo-Zero kits can be used with TruSeq Stranded mRNA kits to create libraries.
Starting Material: Begin RiboZero with 1-5ug total RNA, then take 10-100ng RiboZero product into TruSeq protocol (see second note in "Purify and Fragment mRNA" section of the User Guide).

LT HT
TruSeq Stranded mRNA Catalog ID(s):
RS-122-2101 (48 samples) (Set A: 12 indices)
RS-122-2102 (48 samples) (Set B: 12 indices)
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TruSeq Stranded mRNA Catalog ID(s):
RS-122-2103 (96 samples) (96 indices in plate format)

The index plate is single use per well and cannot undergo more than four freeze/thaw cycles. We strongly recommend processing at least 24 samples at a time

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* Library prep is compatible with all Illumina instruments. Recommended instruments chosen based on typical output and analysis need for selected application.
† Based on 20M reads per sample for RNA profiling and 50M reads per sample for transcriptome analysis. Use twice the number of reads for FFPE or degraded samples.
Project Recommendations
Species Compatibility Test species compatibility with MatchMaker™ Tool
Recommended Illumina Instrument* NextSeq 500 HiSeq 2500 (Dual Flow Cell)
Recommended Samples per run† RNA Profiling: 6–20
Transcriptome Analysis: 2–8		 RNA Profiling: 30–200
Transcriptome Analysis: 12–80
Run Mode Mid Output High Output Rapid Run High Output
Max Recommended Read Length 2x75 bp 2x75 bp 2x75 bp 2x75 bp
Recommended Secondary Analysis
  • TopHat Alignment BaseSpace App
  • Cufflinks Assembly & DE BaseSpace App
  • RNA Express v1.0 BaseSpace App
Recommended Down Stream Analysis
  • Genomatix Pathway
  • iPathway Guide
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For Research Use Only

Not for use in diagnostic procedures (except as specifically noted).

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