TruSeq Stranded Total RNA Ribo-Zero H/M/R Gold

TruSeq Stranded Total RNA Ribo-Zero H/M/R Gold

These kit options include Ribo-Zero H/M/R Gold, which depletes samples of cytoplasmic and mitochondrial ribosomal RNA and preserves strandedness of transcripts. Strand information is useful for detection of antisense transcription, transcript annotation, and increased alignment efficiency. This kit has been explicitly tested on human, mouse, and rat samples. For other species, use the RNA MatchMaker Tool to test Ribo-Zero compatibility in silico.

LT HT
Catalog ID(s):
RS-122-2301 (48 samples) (Set A: 12 indices)
RS-122-2302 (48 samples) (Set B: 12 indices)
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Catalog ID(s):
RS-122-2303 (96 samples) (96 indices in plate format)

The index plate is single use per well and cannot undergo more than four freeze/thaw cycles. We strongly recommend processing at least 24 samples at a time

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Project Recommendations*
Species Compatibility Human, Mouse, Rat
Test other species compatibility with MatchMaker™ Tool
Recommended Illumina Instrument NextSeq 500 HiSeq 2500 (Dual Flow Cell) NovaSeq 6000 (Dual Flow Cell)†
Recommended Samples per run (RNA Profiling / Transcriptome Analysis)‡ 6–20 / 2–8 30–200 / 12–80 160 / 64 320 / 128 768 / 400
Run Mode Mid Output High Output Rapid Run High Output S1§ S2 S4
Max Recommended Read Length 2×75 bp 2×75 bp 2×75 bp 2×75 bp ≤ 2×100 bp
Recommended Secondary Analysis
  • TopHat Alignment BaseSpace App
  • Cufflinks Assembly & DE BaseSpace App
  • RNA Express v1.0 BaseSpace App
Recommended Down Stream Analysis
  • Genomatix Pathway
  • iPathway Guide

* Library prep is compatible with all Illumina instruments. Recommended instruments chosen based on typical output and analysis need for selected application.

† Limited by index combinations (HT). Based on 20M / 50M reads.

‡ Based on 20M reads per sample for RNA profiling and 50M reads per sample for transcriptome analysis. Use twice the number of reads for FFPE or degraded samples.

§ NovaSeq S1 Reagent Kits are not currently available.

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For Research Use Only

Not for use in diagnostic procedures (except as specifically noted).

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