TruSeq Stranded Total RNA Sample Preparation Kits with Ribo-Zero Plant deliver the clearest, most complete view of the plant transcriptome. These kit options include Ribo-Zero Plant, which depletes samples of cytoplasmic, mitochondrial, and chloroplast ribosomal RNA.
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Species Compatibility | Plant | ||||||
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Recommended Illumina Instrument | NextSeq 500 | HiSeq 2500 (Dual Flow Cell) | NovaSeq 6000 (Dual Flow Cell)† | ||||
Recommended Samples per run (RNA Profiling / Transcriptome Analysis)‡ | 6–20 / 2–8 | 30–300 / 12–80 | 160 / 64 | 320 / 128 | 768 / 400 | ||
Run Mode | Mid Output | High Output | Rapid Run | High Output | S1§ | S2 | S4 |
Max Recommended Read Length | 2×75 bp | 2×75 bp | 2×75 bp | 2×75 bp | ≤ 2×100 bp | ||
Recommended Secondary Analysis |
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Recommended Down Stream Analysis |
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* Library prep is compatible with all Illumina instruments. Recommended instruments chosen based on typical output and analysis need for selected application.
† Limited by index combinations (HT). Based on 20M / 50M reads.
‡ Based on 20M reads per sample for RNA profiling and 50M reads per sample for transcriptome analysis. Use twice the number of reads for FFPE or degraded samples.
§ NovaSeq S1 Reagent Kits are not currently available.