TruSeq Stranded Total RNA Ribo-Zero Plant

TruSeq Stranded Total RNA Ribo-Zero Plant

TruSeq Stranded Total RNA Sample Preparation Kits with Ribo-Zero Plant deliver the clearest, most complete view of the plant transcriptome. These kit options include Ribo-Zero Plant, which depletes samples of cytoplasmic, mitochondrial, and chloroplast ribosomal RNA.

LT HT

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Project Recommendations*
Species Compatibility Plant
Recommended Illumina Instrument NextSeq 500 HiSeq 2500 (Dual Flow Cell) NovaSeq 6000 (Dual Flow Cell)
Recommended Samples per run (RNA Profiling / Transcriptome Analysis) 6–20 / 2–8 30–300 / 12–80 160 / 64 320 / 128 768 / 400
Run Mode Mid Output High Output Rapid Run High Output S1§ S2 S4
Max Recommended Read Length 2×75 bp 2×75 bp 2×75 bp 2×75 bp ≤ 2×100 bp
Recommended Secondary Analysis
  • TopHat Alignment BaseSpace App
  • Cufflinks Assembly & DE BaseSpace App
  • RNA Express v1.0 BaseSpace App
Recommended Down Stream Analysis
  • Genomatix Pathway
  • iPathway Guide

* Library prep is compatible with all Illumina instruments. Recommended instruments chosen based on typical output and analysis need for selected application.

† Limited by index combinations (HT). Based on 20M / 50M reads.

‡ Based on 20M reads per sample for RNA profiling and 50M reads per sample for transcriptome analysis. Use twice the number of reads for FFPE or degraded samples.

§ NovaSeq S1 Reagent Kits are not currently available.