The TruSeq Synthetic Long-Read DNA Library Prep kit and accompanying TruSeq Synthetic Long-Read DNA Barcode kit enable preparation of libraries for long-read assembly and phasing analysis. The TruSeq Synthetic Long-Read DNA Library Prep kit provides enough reagents to prepare 4 libraries. Each library requires at least 1 barcode plate. Depending upon the desired long read coverage, additional barcode plates are required for the long-read application.
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| Project Recommendations | ||||
|---|---|---|---|---|
| Species Compatibility | Long Range Assembly: All Species Phasing Analysis: Human |
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| Recommended Illumina Instrument | HiSeq 2500 (Dual Flow Cell) | |||
| Recommended Samples per run | Depends on genome size and desired coverage. Each barcode plate requires 600 Mb of sequencing data. | |||
| Run Mode | Rapid Run | High Output | ||
| Max Recommended Read Length | Up to 2x150 bp | Up to 2x125 bp | ||
| Recommended Secondary Analysis |
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