TruSeq Synthetic Long-Read

TruSeq Synthetic Long-Read

The TruSeq Synthetic Long-Read DNA Library Prep kit and accompanying TruSeq Synthetic Long-Read DNA Barcode kit enable preparation of libraries for long-read assembly and phasing analysis. The TruSeq Synthetic Long-Read DNA Library Prep kit provides enough reagents to prepare 4 libraries. Each library requires at least 1 barcode plate. Depending upon the desired long read coverage, additional barcode plates are required for the long-read application.

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Project Recommendations
Species Compatibility Long Range Assembly: All Species
Phasing Analysis: Human
Recommended Illumina Instrument HiSeq 2500 (Dual Flow Cell)
Recommended Samples per run Depends on genome size and desired coverage. Each barcode plate requires 600 Mb of sequencing data.
Run Mode Rapid Run High Output
Max Recommended Read Length Up to 2x150 bp Up to 2x125 bp
Recommended Secondary Analysis
  • TruSeq Long-Read Assembly BaseSpace App
  • TruSeq Phasing Analysis BaseSpace App