Comparison Table

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  AmpliSeq for Illumina Custom DNA Panel Nextera Rapid Capture Custom Enrichment Nextera XT DNA
Description Leveraging high-performance AmpliSeq chemistry, researchers can use the AmpliSeq for Illumina Custom assay to focus their studies on specific genes, regions, or variants of interest with high accuracy. Custom workflow enabling enrichment of targeted genomic content. Design completely custom panels, or add-on to existing Illumina designed panels or previously ordered custom projects. Allows the preparation of sequencing-ready libraries for amplicons in 90 minutes, with only 15 minutes of hands-on time with small DNA input.
Design Illumina DesignStudio Software is powerful and easy to use tool for designing amplicons as hotspots or genes to target user’s region of interest. Illumina DesignStudio Software is a powerful and easy to use tool for designing probes to target user’s region of interest. Third-party vendor designed user primers to target amplicons that are at least 300bp in length.
Preloaded Genomes Human, cow, pig, maize, mouse, chicken, sheep, rice, soybean, and tomato Human N/A
Content Range Up to 5Mb 0.5–15Mb 300bp–20Mb
Kit Size 750 or 3000 rxn 48, 96, or 288 samples 24 or 96 samples
Input Amount 1–100ng; 10ng per pool recommended 50 ng DNA 1 ng DNA
Multiplexing Up to 96 plex Up to 96 plex Up to 96 plex
Method Amplicon Enrichment Amplicon
FFPE Compatible Yes No No
For Research Use Only

Not for use in diagnostic procedures (except as specifically noted).

Innovative technologies

At Illumina, our goal is to apply innovative technologies to the analysis of genetic variation and function, making studies possible that were not even imaginable just a few years ago. It is mission critical for us to deliver innovative, flexible, and scalable solutions to meet the needs of our customers. As a global company that places high value on collaborative interactions, rapid delivery of solutions, and providing the highest level of quality, we strive to meet this challenge. Illumina innovative sequencing and array technologies are fueling groundbreaking advancements in life science research, translational and consumer genomics, and molecular diagnostics.

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