Comparison Table
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| TruSeq DNA Exome | Nextera DNA Exome | AmpliSeq for Illumina Exome Panel | |
|---|---|---|---|
| Description | The most efficient Exome workflow focused on ≥98% of RefSeq, CCDS, and Ensembl coding content. | The most cost effective Exome workflow focused on ≥98% of RefSeq, CCDS, and Ensembl coding content. | Prepare 8 exome libraries in <7.5 hours with <1.5 hours hands on time |
| Design | Human Exome, Illumina designed, coding regions | Human Exome, Illumina designed, coding regions | Human protein-coding regions of the genome |
| Targeted Region | 45 Mb | 45 Mb | 59Mb |
| Final Multiplexing | Up to 96 | Up to 96 | Up to 96 |
| Assay Time | 2.5 days | 2 days | 7.5 hours |
| Fragmentation Method | Mechanical | Transposase-based | Amplicon |
| On-Target % | >80% | >75% | >80% |
| % Coverage at 20x | >90% | >85% | >90% |