Comparison Table

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  Nextera XT Nextera DNA Flex
Intended Use Allows the preparation of sequencing-ready libraries for small genomes, amplicons, and plasmids in less than 90 minutes with only 15 minutes of hands-on time. Amplicon inputs are required be at least 300 bp in length. Illumina’s fastest and easiest workflow from sample to normalized library.
Input Quantity 1ng 1–500ng
Assay Time ~1.5 hours 3–3.5 hours (from blood/saliva DNA extraction to normalized library)
Multiplexing 384 dual index 96 dual index
Fragmentation Enzymatic Enzymatic
Target Insert Size 300bp–1.5kb ~500bp
Bisulfite Compatibility No No
For Research Use Only

Not for use in diagnostic procedures (except as specifically noted).

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