Comparison Table
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| TruSeq Targeted RNA Expression | TruSight RNA Pan-Cancer Panel | TruSight RNA Fusion Panel | TruSight Tumor 170 | |
|---|---|---|---|---|
| Description | Customizable mid- to high-plex gene expression analysis with user-defined panels of 12-1000 assays. | Comprehensive assessment of cancer-related RNA transcripts enabling detection of gene fusions, variants, and gene expression changes in all cancer types. | Gene fusion detection with known and novel partners in 507 fusion associated genes. Simplified analysis solution reports all detected fusions. | Comprehensive somatic mutation detection in solid tumors. Uses variant calling information from both DNA and RNA. |
| Design |
|
Pan-Cancer Panel with 1,385 targeted genes and 21,043 targeted exonic regions. Includes 507 genes involved in cancer gene fusions. | Gene fusion panel targeting 507 cancer-associated fusion genes and 7690 exons. | Fixed set of probes that enrich for full coding sequences of 170 genes. Single nucleotide variants, small insertions, and deletions are called in 151 genes; amplifications are called in 59 genes; fusions and splice variants are called in 55 genes. |
| Method | Amplicon | Enrichment | Enrichment | Enrichment |
| Input (total RNA) | 50 ng for high quality RNA ≥ 200 ng for degraded/FFPE RNA |
10 ng for high quality RNA 20–100 ng for FFPE RNA |
10 ng for high quality RNA 20–100 ng for FFPE RNA |
40–120 ng FFPE DNA 40–80 ng FFPE RNA |
| FFPE Compatible | Yes | Yes | Yes | Yes |