AmpliSeq for Illumina BRCA Panel
AmpliSeq for Illumina BRCA Panel
The AmpliSeq for Illumina BRCA Panel is a targeted resequencing assay designed for detecting somatic and germline mutations across all exonic regions and the flanking intronic sequences of BRCA1 and BRCA2. BRCA1 and BRCA2 are human tumor suppressor genes that, when carrying specific mutations, have been implicated in an increased risk for breast and ovarian cancers. The BRCA panel is part of a streamlined workflow that includes PCR-based library preparation, Illumina sequencing by synthesis (SBS) chemistry and next-generation sequencing (NGS) technology, and automated analysis. Requiring as little as 1 ng high quality DNA per pool, the two-pool panel can be used with low-quality and low-quantity samples, including formalin-fixed, paraffin-embedded (FFPE) tissues.
- Panel:
- 20019168 AmpliSeq for Illumina BRCA Panel, 24 Reactions
- Library Prep:
- 20019101 AmpliSeq for Illumina Library PLUS, 24 Reactions
- 20019102 AmpliSeq for Illumina Library PLUS, 96 Reactions
- 20019103 AmpliSeq for Illumina Library PLUS, 384 Reactions
- Indexes:
- 20019105 AmpliSeq for Illumina CD Indexes Set A, 96 Indexes, 96 Reactions
| Species Compatibility | Human | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Recommended Illumina Instrument* | MiniSeq | MiSeq | iSeq 100 | |||||||
| Recommended Samples per run | Mid Output | High Output | v2 Nano | v2 Micro | v2 | v3 | 12† | 96** | ||
| 24 | 80 | 3† | 32** | 12 | 96** | 48† | 80† | |||
| Max Recommended Read Length | 2×150 | Up to 2×150 | ||||||||
| Recommended Secondary Analysis | DNA Amplicon App, DNA Amplicon Module | |||||||||
| Recommended Down Stream Analysis | Variant Interpreter | |||||||||
* Library prep is compatible with all Illumina instruments. Recommended instruments chosen based on typical output and analysis need for selected application.
† Assumes minimum coverage of 500×
** Assumes minimum coverage of 50×
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