AmpliSeq for Illumina Cancer Hotspot Panel v2

AmpliSeq for Illumina Cancer Hotspot Panel v2

The AmpliSeq for Illumina Cancer Hotspot Panel v2 is a targeted resequencing assay for detecting somatic mutations across the hotspot regions of 50 genes with known acludes AmpliSeq for Illumina PCR-based library preparation, Illumina sequencing by synthesis (SBS) chemistry and next-generation sequencing (NGS) technology, and automated analysis with cloud-based BaseSpace Sequence Hub and BaseSpace Variant Interpreter, or local, on-instrument options. Starting with as little as 10 ng input DNA, the panel enables highly sensitive mutation detection within multiple cancer types, including lung, colon, breast, ovarian, melanoma, and prostate. The low-input requirement enables use with various sample types, including formalin-fixed, paraffin-embedded (FFPE) tissues.ssociations to cancer. The Cancer Hotspot Panel is part of a streamlined workflow that includes AmpliSeq for Illumina PCR-based library preparation, Illumina sequencing by synthesis (SBS) chemistry and next-generation sequencing (NGS) technology, and automated analysis with cloud-based BaseSpace Sequence Hub and BaseSpace Variant Interpreter, or local, on-instrument options. Starting with as little as 10 ng input DNA, the panel enables highly sensitive mutation detection within multiple cancer types, including lung, colon, breast, ovarian, melanoma, and prostate. The low-input requirement enables use with various sample types, including formalin-fixed, paraffin-embedded (FFPE) tissues.

Panel:
20019161 AmpliSeq for Illumina Cancer Hotspot Panel v2, 24 Reactions
Library Prep:
20019101 AmpliSeq for Illumina Library PLUS, 24 Reactions
20019102 AmpliSeq for Illumina Library PLUS, 96 Reactions
20019103 AmpliSeq for Illumina Library PLUS, 384 Reactions
Indexes:
20019105 AmpliSeq for Illumina CD Indexes Set A, 96 Indexes, 96 Reactions
Project Recommendations
Species Compatibility Human
Recommended Illumina Instrument* MiniSeq MiSeq iSeq 100
Recommended Samples per run† Mid Output High Output v2 Nano v2 Micro v2 v3 16
32 96 4 16 60 96
Max Recommended Read Length 2×150
Recommended Secondary Analysis DNA Amplicon App, DNA Amplicon Module
Recommended Down Stream Analysis Variant Interpreter

* Library prep is compatible with all Illumina instruments. Recommended instruments chosen based on typical output and analysis need for selected application.

† Assumes minimum coverage of 500×

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For Research Use Only

Not for use in diagnostic procedures (except as specifically noted).

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