AmpliSeq for Illumina Comprehensive Cancer Panel

AmpliSeq for Illumina Comprehensive Cancer Panel

The AmpliSeq for Illumina Comprehensive Cancer Panel provides a targeted resequencing solution for analyzing somatic mutations across 409 genes with known associations. The Comprehensive Cancer panel is part of a streamlined workflow that includes PCR-based library preparation, Illumina sequencing by synthesis (SBS) chemistry and next-generation sequencing (NGS) technology, and automated analysis. It requires as little as 1 ng high quality DNA or 10 ng from DNA from low-quality samples per pool, making it compatible with various sample types, including formalin-fixed, paraffin-embedded (FFPE) tissues to multiple cancer types, including lung, colon, breast, ovarian, melanoma, and prostate.

Panel:
20019160 AmpliSeq for Illumina Comprehensive Cancer Panel, 24 Reactions
Library Prep:
20019101 AmpliSeq for Illumina Library PLUS, 24 Reactions
20019102 AmpliSeq for Illumina Library PLUS, 96 Reactions
20019103 AmpliSeq for Illumina Library PLUS, 384 Reactions
Indexes:
20019105 AmpliSeq for Illumina CD Indexes Set A, 96 Indexes, 96 Reactions
Project Recommendations
Species Compatibility Human
Recommended Illumina Instrument* NextSeq
Recommended Samples per run Mid Output High Output
4 12
Max Recommended Read Length 2×150
Recommended Secondary Analysis DNA Amplicon App, DNA Amplicon Module
Recommended Down Stream Analysis Variant Interpreter

* Library prep is compatible with all Illumina instruments. Recommended instruments chosen based on typical output and analysis need for selected application.

† Assumes minimum coverage of 500×

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