The AmpliSeq for Illumina Comprehensive Cancer Panel provides a targeted resequencing solution for analyzing somatic mutations across 409 genes with known associations. The Comprehensive Cancer panel is part of a streamlined workflow that includes PCR-based library preparation, Illumina sequencing by synthesis (SBS) chemistry and next-generation sequencing (NGS) technology, and automated analysis. It requires as little as 1 ng high quality DNA or 10 ng from DNA from low-quality samples per pool, making it compatible with various sample types, including formalin-fixed, paraffin-embedded (FFPE) tissues to multiple cancer types, including lung, colon, breast, ovarian, melanoma, and prostate.
Species Compatibility | Human | |
---|---|---|
Recommended Illumina Instrument* | NextSeq | |
Recommended Samples per run† | Mid Output | High Output |
4 | 12 | |
Max Recommended Read Length | 2×150 | |
Recommended Secondary Analysis | DNA Amplicon App, DNA Amplicon Module | |
Recommended Down Stream Analysis | Variant Interpreter |
* Library prep is compatible with all Illumina instruments. Recommended instruments chosen based on typical output and analysis need for selected application.
† Assumes minimum coverage of 500×
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