AmpliSeq for Illumina Focus Panel
AmpliSeq for Illumina Focus Panel
The AmpliSeq for Illumina Focus Panel is a targeted resequencing assay for biomarker analysis of 52 genes with known relevance to solid tumors. Using the Focus Panel, researchers can analyze both DNA and RNA concurrently. The Focus Panel is part of a streamlined workflow that includes AmpliSeq for Illumina PCR-based library preparation, Illumina sequencing by synthesis (SBS) next-generation sequencing (NGS) technology, and automated analysis. Starting with as little as 1 ng high-quality DNA and RNA (recommend 10 ng DNA and RNA from FFPE tissue), the panel enables the study of genes associated with multiple cancer types, including lung, colon, breast, ovarian, melanoma, and prostate.
- Panel:
- 20019164 AmpliSeq for Illumina Focus Panel, 24 Reactions
- Library Prep:
- 20019101 AmpliSeq for Illumina Library PLUS, 24 Reactions
- 20019102 AmpliSeq for Illumina Library PLUS, 96 Reactions
- 20019103 AmpliSeq for Illumina Library PLUS, 384 Reactions
- Indexes:
- 20019105 AmpliSeq for Illumina CD Indexes Set A, 96 Indexes, 96 Reactions
| Species Compatibility | Human | ||||||
|---|---|---|---|---|---|---|---|
| Recommended Illumina Instrument* | iSeq 100 | MiniSeq | MiSeq | ||||
| Recommended Samples per run† | 8 | Mid Output | High Output | v2 Micro | v2 | v3 | |
| 16 | 48 | 8 | 30 | 48 | |||
| Max Recommended Read Length | 2×150 | ||||||
| Recommended Secondary Analysis | DNA Amplicon App, DNA Amplicon Module, RNA Amplicon App, RNA Amplicon Module | ||||||
| Recommended Down Stream Analysis | Variant Interpreter | ||||||
* Library prep is compatible with all Illumina instruments. Recommended instruments chosen based on typical output and analysis need for selected application.
† Assumes minimum coverage of 500×
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