The AmpliSeq for Illumina Focus Panel is a targeted resequencing assay for biomarker analysis of 52 genes with known relevance to solid tumors. Using the Focus Panel, researchers can analyze both DNA and RNA concurrently. Key features include:
The Focus Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) next-generation sequencing (NGS) technology, and automated analysis.
This ready-to-use panel saves you the time and effort of identifying targets, designing primers, and optimizing panels.
Instrument | Recommended Number of Samples | Read Length |
---|---|---|
iSeq 100 System | 8 samples per run (assumes minimum coverage of 500×) | 2 × 150 bp |
MiniSeq System | Samples per run: mid output: 16, high output: 48 (assumes minimum coverage of 500×) | 2 × 150 bp |
MiSeq System | Samples per run (by reagent kit version): v2 micro: 8, v2: 30, v3: 48 (assumes minimum coverage of 500×) | 2 × 150 bp |
AmpliSeq for Illumina Focus Panel | AmpliSeq for Illumina Comprehensive Panel v3 | AmpliSeq for Illumina Cancer Hotspot Panel v2 | TruSight Tumor 15 | |
---|---|---|---|---|
Assay Time | 5-6 hours (library prep only; doesn't include library quantification, normalization, or pooling time) | 5-6 hours (library prep only; doesn't include library quantification, normalization, or pooling time) | 5 hours (library prep only; doesn't include library quantification, normalization, or pooling time) | 7 hours |
Cancer Type | Solid Tumor | Solid Tumor | Pan-Cancer, Solid Tumor | Solid Tumor |
Content Specifications | DNA and RNA targets for 52 oncogenes | DNA and RNA targets for 161 oncogenes | Hotspot regions of 50 genes with known associations to cancer | Amplifies 250 amplicons from 15 genes associated with solid tumors |
Description | Somatic analysis research on 52 genes associated with solid tumor cancers. | Somatic analysis research on hotspot and full-length targets of genes* associated with solid tumor cancers. | Somatic analysis research into hotspot regions of 50 cancer-related genes. | Focused panel to study relevant solid tumor somatic variants in 15 genes using a simple workflow. |
Hands-On Time | < 1.5 hours | < 1.5 hours | < 1.5 hours | 3.5 hours |
Input Quantity | 1–100 ng (10 ng recommended per pool) | 1–100 ng (10 ng recommended per pool) | 1–100 ng (10 ng recommended per pool) | 20 ng |
Method | Amplicon Sequencing, Targeted DNA Sequencing, Targeted RNA Sequencing | Amplicon Sequencing, Targeted DNA Sequencing, Targeted RNA Sequencing | Amplicon Sequencing, Targeted DNA Sequencing | Amplicon Sequencing, Targeted DNA Sequencing |
Multiplexing | 96 dual index combinations | 96 dual index combinations | 96 dual index combinations | 1-24-plex |
Nucleic Acid Type | DNA, RNA | DNA, RNA | DNA | DNA |
Specialized Sample Types | FFPE Tissue | FFPE Tissue | FFPE Tissue | FFPE Tissue |
Species Category | Human | Human | Human | Human |
AmpliSeq for Illumina Oncology Panels on the iSeq 100 System
Application Note | PDF < 1 MB
Analytical performance of the AmpliSeq for Illumina Focus Panel with FFPE samples
Application Note | PDF < 1 MB
Automated Solutions for AmpliSeq for Illumina Sequencing Panels
Application Note | HTML
Ampliseq for Illumina Focus Panel Data Sheet
Data Sheet | HTML
AmpliSeq for Illumina Focus Panel Gene List
product_file | EXCEL < 1 MB
Application Note | PDF | 5 versions
AmpliSeq for Illumina Focus Panel Documentation
Illumina Adapter Sequences Documentation
AmpliSeq for Illumina Focus Panel Consumables & Equipment Documentation
AmpliSeq for Illumina Focus Panel Checklist Documentation
AmpliSeq for Illumina Direct FFPE DNA Kit Reference Guide Documentation
AmpliSeq for Illumina Focus Panel Reference Guide Documentation
Index Adapters Pooling Guide Documentation
Custom Protocol Selector
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