Comparison Table
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| TruSeq DNA PCR-Free | TruSeq Nano DNA | Nextera DNA | |
|---|---|---|---|
| Intended Use | Leverages a workflow similar to TruSeq DNA, but without PCR. Is a simple, all-inclusive prep for whole genome sequencing with the industry best genome coverage and ability to sequence through challenging regions of the genome. | A low-input method that delivers high genome coverage quality and reduced bias. | Sequencing's fastest and easiest workflow delivering sequencing ready libraries in 90 minutes. |
| Input Quantity | 1ug/2ug | LT/HT: 100ng/200ng | 50ng |
| Assay Time | ~5 hours | LT/HT: ~5.5 hours | ~1.5 hours |
| Hands-on Timea | ~5 hours | LT/HT: ~4 hours | ~15 minutes |
| Multiplexing | LT Kit: 24
HT Kit: 96 |
LT Kit: 24
HT Kit: 96 |
96plex |
| Fragmentation | Mechanical | Enzymatic | |
| Target Insert Size | 350bp or 550bp | 300bp – 1.5kb | |
| Bisulfite Compatibility | LT Kit: Yes, adapters are methylated
HT Kit: No |
No | |
| Enrichment Compatibility | No, please see Nextera Rapid Capture Products. | ||
a Hands-on time is for 16 libraries from library prep through normalization.