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Stranded Total RNA Species Compatibility

Species Compatibility Details

Our stranded total RNA library prep solutions are designed for human, mouse, rat and plant sequences, but may be applicable to a variety of eukaryotic species. The following tables list organisms that have been laboratory tested and are fully supported by Illumina, tested in silico, or reported to work by our customers. Illumina cannot guarantee rRNA removal based on customer-reported results.

Benefits of RNA Sequencing

RNA-Seq uses next-generation sequencing to analyze expression across the transcriptome, enabling researchers to detect known or novel features and quantify RNA.

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Illumina Stranded Total RNA Prep with Ribo-Zero Plus
Sample rRNAs Targeted
Human cytoplasmic rRNAs 28S, 18S, 5.8S, 5S
Human mitochondrial rRNAs 12S, 16S
Human beta globin transcripts HBA1, HBA2, HBB, HBG1, HBG2
Mouse & Rat rRNA 16S, 28S
Gram(-) Bacterial rRNAs E. coli: 5S, 16S, 23S
Gram(+) Bacterial rRNAs Bacillus subtilis: 5S, 16S, 23S

TruSeq Stranded Total RNA-with Ribo-Zero Human/Mouse/Rat
TruSeq Stranded Total RNA-with Ribo-Zero Gold
Organism Laboratory Tested In silico Tested Customer Reported Comments
Bee (Apis mellifera)       Use highest quality RNA possible.
Chicken (Gallus gallus)       Removes 5S, 18S and 28S rRNAs. Mitochondrial rRNAs not tested.
Ciona intestinalis       Removes 18S rRNA. Other rRNAs not tested.
Colletotrichum higginsianum       Will remove 28S and 5.8S rRNA. Will not remove 5S rRNA. 18S rRNA not evaluated.
Copepod species       Removes 28S and 18S rRNAs. Other rRNAs were not evaluated.
Cow        
Dictyostelium discoideum       Removes 28S and 18S rRNA from high quality RNA. Will not remove small rRNAs.
Dog        
Ferret (Mustela putorius)        
Finch (Carpodacus mexicanus)       Removes 5S and 18S rRNA. Will not remove 18S rRNA. Mitochondrial rRNAs not tested.
Flour beetle (Tribolium castaneum)        
Frog (Xenopus)        
Fruit Fly (Drosophila)       Will not remove 5S rRNA or 'right' fragment of 28S rRNA.
Fungus (Aspergillus niger)       300 nt region of 18S rRNA will not be removed from a degraded sample. Use highest quality RNA possible.
Fungus (Basidiomycete)       Use highest quality RNA possible.
Fungus (Candida albicans)        
Fungus (Cryptococcus neoformans)       Will not remove 5.8S or 5S rRNAs. Use highest quality RNA possible.
Fungus (Yarrowia lipolytica)       Will not remove 5.8S or 5S rRNAs. Use highest quality RNA possible.
Horse (Equine)        
Human       All tissues except blood; use TruSeq Stranded Total RNA-with Ribo-Zero Globin for blood samples.
Insect (Rhodnius prolixus)       Use highest quality RNA possible. Mitochondrial rRNA not removed.
Lamprey       Use highest quality RNA possible.
Marine sponge (Crambe crambe)       Use highest quality RNA possible.
Monkey (Macaque; Cynomolgus macaques)        
Monkey (African green; Chlorocebus sabaeus)        
Monkey (Rhesus; Macaca mulatta)        
Mosquitoes (Anopheles gambiae)       Removes 18S rRNA. Will not remove 5S, 5.8S, and 12S, 16S mitochondrial rRNA. 28S rRNA not tested.
Mouse       All tissues except blood; use TruSeq Stranded Total RNA-with Ribo-Zero Globin for blood samples.
Nematode (C. elegans)       Use highest quality RNA possible.
Newt (Notophthalmus viridescens)       May not remove 5.8S or 5S rRNAs. Use highest quality RNA possible.
Plasmodium falciparum       Will not remove 5.8S or 5S rRNAs. Use highest quality RNA possible.
Poeciliid fishes       Removes 28S and 18S rRNA. May not remove all 5S rRNA.
Rat       All tissues except blood; use TruSeq Stranded Total RNA-with Ribo-Zero Globin for blood samples.
Salmon (Salmo salar)       Removes 18S, 5.8S, and 5S rRNAs. Will not remove 28S rRNA. Mitochondrial rRNAs were not evaluated.
Sea anemone (Nematostella vectensis)       Use highest quality RNA possible.
Swine       Removes 28S, 18S, 5.8S and 5S rRNAs.
Toxoplasma gondii       Use high quality RNA.
Trypanosoma brucei       Will remove 28S and 18S rRNA. Other rRNA were not evaluated.
Welsh Onion (Allium fistulosum)       Removes 18S rRNA. Other rRNAs not tested.
Zebrafish (Danio rerio)        

TruSeq Stranded Total RNA-with Ribo-Zero Globin
Organism Laboratory Tested In silico Tested Customer Reported Comments
Human       Blood samples only; for all other tissues use TruSeq Stranded Total RNA-with Ribo-Zero Human/Mouse/Rat or TruSeq Stranded Total RNA-with Ribo-Zero Gold
Mouse       Blood samples only; for all other tissues use TruSeq Stranded Total RNA-with Ribo-Zero Human/Mouse/Rat or TruSeq Stranded Total RNA-with Ribo-Zero Gold
Rat       Blood samples only; for all other tissues use TruSeq Stranded Total RNA-with Ribo-Zero Human/Mouse/Rat or TruSeq Stranded Total RNA-with Ribo-Zero Gold

TruSeq Stranded Total RNA-with Ribo-Zero Plant
Organism Laboratory Tested In silico Tested Customer Reported Comments
Arabidopsis       Compatible with leaves, seeds and roots.
Chickpea (Cicer arietinum)       Removes 4.5S, 16S, 23S chloroplast rRNA and 18S cytoplasmic rRNA. Will not remove 5S chloroplast and 5S cytoplasmic rRNA. Other rRNAs not tested.
Corn       Compatible with leaves, seeds and roots.
Cotton        
Eukaryotic green algae (Chlamydomonas)       Use highest quality RNA possible.
Grapevine        
Japanese honeysuckle (Lonicera japonica)        
Maize       Compatible with leaves, seeds and roots.
Mirror bush (Coprosma repens)        
Moss (Physcomitrella)        
Prorocentrum minimum       Removes 18S and 28S rRNA. Other rRNAs not tested.
Rice       Compatible with leaves, seeds and roots.
Soybean       Compatible with leaves, seeds and roots.
Tomato (Solanum lycopersicum)        
Vietnamese coriander (Persicaria odorata)        
Water clover (Marsilea vistita)        
Welsh onion       Use highest quality RNA possible.
Wheat       Compatible with leaves, seeds and roots.

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