TruSeq Stranded Total RNA with Ribo-Zero Globin delivers a clear and comprehensive view of the transcriptome from blood-derived total RNA, with fast, efficient sequencing library preparation.
These kits couple the benefits of Ribo-Zero ribosomal RNA reduction chemistry with RNA sequencing (RNA-Seq) technology for whole-transcriptome analysis of human, mouse, or rat samples. This technology provides:
Whole-transcriptome analysis of blood-derived RNA requires the removal of two forms of abundant RNA—ribosomal RNA (both cytoplasmic and mitochondrial) as well as globin mRNA, which is present in high levels in whole blood.
Traditional removal methods require two independent steps. This means the need for additional reagents, a longer workflow, and more input RNA lost. TruSeq Stranded Total RNA with Ribo-Zero Globin leverages Ribo-Zero chemistry to efficiently remove both forms of abundant RNA in a single, rapid step.
Read an assessment of our rRNA removal process
|Instrument||Recommended Number of Samples||Read Length|
|NextSeq 550 System||RNA Profiling: 6-20 samples per run (based on 20 million reads per sample)
Transcriptome Analysis: 2-8 samples per run (based on 50 million reads per sample)
|2 x 75 bp
2 x 75 bp
|NovaSeq 6000 System||RNA Profiling (samples per run, dual flow cell): S1: 160, S2: 320, S4: 768. Limited by index combinations (dual). Based on 20 million reads.
Transcriptome Analysis (samples per run, dual flow cell): S1: 64, S2: 128, S4: 400. Limited by index combinations (dual). Based on 50 million reads.
|≤ 2 × 100 bp
≤ 2 × 100 bp
|TruSeq Stranded Total RNA with Ribo-Zero Globin||Illumina Stranded Total RNA Prep with Ribo-Zero Plus or Ribo-Zero Plus Microbiome|
|Assay Time||11.5 hours||~7 hours|
|Automation Capability||Liquid Handling Robots||Liquid Handling Robots|
|Content Specifications||Captures coding RNA plus multiple forms of non-coding RNA||Captures coding RNA plus multiple forms of non-coding RNA|
|Description||For whole-transcriptome sequencing studies of blood-derived RNA. Depletes cytoplasmic and mitochondrial rRNA plus globin mRNA.||For whole-transcriptome sequencing studies of multiple sample types. Includes Ribo-Zero plus module for single-tube depletion of abundant transcripts from multiple species|
|Hands-On Time||5.5 hours||< 3 hours|
|Input Quantity||0.1 – 1 ug high-quality purified total RNA from blood||1-1000 ng total RNA for standard-quality RNA samples. 10ng total RNA minimum recommended for optimal performance and FFPE samples|
|Mechanism of Action||Bead-based rRNA depletion, cDNA synthesis, and PCR||Enzymatic rRNA depletion, ligation-based addition of adapters and indexes|
|Method||Whole-Transcriptome Sequencing||Metatranscriptome Sequencing, Whole-Transcriptome Sequencing|
|Multiplexing||High-throughput kits: Prepare 96 indexes samples using either combinatorial dual indexes or unique dual indexes (available soon), Low-throughput kits: Pool up to 12 samples. Or pool up to 24 samples with sets A and B together||Up to 384 Unique Dual Indexes (UDIs)|
|Specialized Sample Types||Blood|
|Species Category||Human, Mouse, Rat||Bacteria, Human, Mouse, Rat|
|Species Details||rRNA Removal: Depletes human, mouse, and rat rRNA (cytoplasmic and mitochondrial) and globin mRNA||Ribo-Zero Plus included for depletion of abundant transcripts from multiple species including: human cytoplasmic & mitochondria rRNA, mouse rRNA, rat rRNA, bacteria Gram +/- rRNA, human beta globin transcripts|
The product configuration for Cat. No. RS-122-2501, RS-122-2502, and RS-122-2503 has changed. In the new configuration, the library preparation and index adapters can be purchased separately. The list of components available for individual purchase can be found in the “Select Product(s)” section.
Technical replicates of FFPE tissue show high concordance, indicating robust library prep performance. Axes are log2(FPKM). R2 value is shown.
TruSeq Stranded Total RNA gives excellent coverage across the top 1,000 expressed transcripts in both fresh-frozen (FF, top) and FFPE (bottom) tumor and matched normal breast tissue, with > 98% aligned stranded reads. X-axis: position along transcript, Y-axis = percent coverage of combined reads.
Data Sheet | PDF | 5 versions
Robust low-input RNA sequencing with TruSeq Stranded Total RNA Library Prep Gold
Application Note | HTML
TruSeq Stranded Total RNA With Illumina Ribo-Zero Plus rRNA Depletion Reference Guide Documentation
TruSeq Stranded Total RNA Dual Index Product Insert Documentation
Sequencing Library qPCR Quantification Guide Documentation
TruSeq Stranded Total RNA Reference Guide Documentation
TruSeq Stranded Total RNA Documentation
TruSeq Stranded Total RNA Sample Prep Guide Documentation
Illumina Experiment Manager User Guide Documentation
TruSeq HT Dual Index Quick Reference Card Documentation
TruSeq Sample Prep Best Practices Guide Documentation
TruSeq Stranded Total RNA Sample Prep HS EUC and LTF Documentation
TruSeq Stranded Total RNA Checklist Documentation
TruSeq Stranded Total RNA Sample Preparation LS EUC and LTF Documentation
TruSeq Stranded Total RNA Consumables & Equipment Documentation
TruSeq Stranded Total RNA Sample Preparation User-Supplied Consumables and Equipment Documentation
TruSeq Stranded Total RNA Support - Documentation & Literature
Custom Protocol Selector
Generates customized, end-to-end instructions
Limited Use Label License: The TruSeq Stranded Total RNA with Ribo-Zero Globin Workflow product and its use are the subject of one or more issued and/or pending U.S. and foreign patent applications owned by Max Planck Gesellschaft, exclusively licensed to New England Biolabs, Inc. and sublicensed to Illumina, Inc. The purchase of this product from Illumina, Inc., its affiliates, or its authorized resellers and distributors conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product by the buyer (whether the buyer is an academic or for profit entity). The purchase of this product does not convey a license under any claims in the foregoing patents or patent applications direct to producing the product. The buyer cannot sell or otherwise transfer this product or its components to a third party or otherwise use the product for the following COMMERICAL PURPOSES: (I) use of the product or its components in manufacturing; or (2) use of the product or its components for therapeutic or prophylactic purposes in human or animals.