Achieve unparalleled study flexibility and single-day prep with our new Illumina Stranded Total RNA Prep. Use code RNA25 at checkout to receive 25% off your first order, now through the end of 2020.* 

*Offer valid for library prep kit only. Index adapters and panels are not included in this promotion.

TruSeq Stranded Total RNA with Ribo-Zero Globin

Prepare whole-transcriptome sequencing libraries from blood-derived RNA with an efficient workflow that removes ribosomal RNA and globin mRNA in a single step.Read More...
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Library Prep

TruSeq® Stranded Total RNA Library Prep Globin (48 Samples)

20020612

Price
 
 

TruSeq® Stranded Total RNA Library Prep Globin (96 Samples)

20020613

Price
 
 

Index Adapters

TruSeq RNA CD Index Plate (96 Indexes, 96 Samples)

20019792

Price
 
 

TruSeq RNA Single Indexes Set A (12 Indexes, 48 Samples)

20020492

Price
 
 

TruSeq RNA Single Indexes Set B (12 Indexes, 48 Samples)

20020493

Price
 
 

IDT for Illumina – TruSeq RNA UD Indexes (24 Indexes, 96 Samples)

20020591

Price
 
 

IDT for Illumina – TruSeq RNA UD Indexes (96 Indexes, 96 Samples)

20022371

Price
 
 

Product Highlights

TruSeq Stranded Total RNA with Ribo-Zero Globin delivers a clear and comprehensive view of the transcriptome from blood-derived total RNA, with fast, efficient sequencing library preparation.

High-quality whole-transcriptome analysis with precise strand information

These kits couple the benefits of Ribo-Zero ribosomal RNA reduction chemistry with RNA sequencing (RNA-Seq) technology for whole-transcriptome analysis of human, mouse, or rat samples. This technology provides:

  • Uniform coverage and precise measurement of strand orientation
  • High-confidence discovery of alternative transcripts, antisense expression, and allele-specific expression
  • Discovery across both coding and multiple forms of noncoding RNA

View Species Compatibility Tables

Efficient ribosomal RNA and globin mRNA removal in a single step

Whole-transcriptome analysis of blood-derived RNA requires the removal of two forms of abundant RNA—ribosomal RNA (both cytoplasmic and mitochondrial) as well as globin mRNA, which is present in high levels in whole blood.

Traditional removal methods require two independent steps. This means the need for additional reagents, a longer workflow, and more input RNA lost. TruSeq Stranded Total RNA with Ribo-Zero Globin leverages Ribo-Zero chemistry to efficiently remove both forms of abundant RNA in a single, rapid step.

Read an assessment of our rRNA removal process

Automation-friendly workflow

Find automation vendors with compatible robotic systems

Frequently Purchased Together

Specifications

Project Recommendations

Instrument Recommended Number of Samples Read Length
NextSeq 550 System RNA Profiling: 6-20 samples per run (based on 20 million reads per sample)
Transcriptome Analysis: 2-8 samples per run (based on 50 million reads per sample)
2 x 75 bp
2 x 75 bp
HiSeq 2500 System RNA Profiling: 30-200 samples per run (dual flow cell; based on 20 million reads per sample)
Transcriptome Analysis: 12-80 samples per run (dual flow cell; based on 50 million reads per sample)
2 x 75 bp
2 x 75 bp
NovaSeq 6000 System RNA Profiling (samples per run, dual flow cell): S1: 160, S2: 320, S4: 768. Limited by index combinations (dual). Based on 20 million reads.
Transcriptome Analysis (samples per run, dual flow cell): S1: 64, S2: 128, S4: 400. Limited by index combinations (dual). Based on 50 million reads. 
≤ 2 × 100 bp
≤ 2 × 100 bp

Product Comparison

TruSeq Stranded Total RNA with Ribo-Zero Globin Illumina Stranded Total RNA Prep with Ribo-Zero Plus
Assay Time 11.5 hours ~7 hours
Automation Capability Liquid Handling Robots Liquid Handling Robots
Content Specifications Captures coding RNA plus multiple forms of non-coding RNA Captures coding RNA plus multiple forms of non-coding RNA
Description For whole-transcriptome sequencing studies of blood-derived RNA. Depletes cytoplasmic and mitochondrial rRNA plus globin mRNA. For whole-transcriptome sequencing studies of multiple sample types. Includes Ribo-Zero plus module for single-tube depletion of abundant transcripts from multiple species
Hands-On Time 5.5 hours < 3 hours
Input Quantity 0.1 – 1 ug high-quality purified total RNA from blood 1-1000 ng total RNA for standard-quality RNA samples. 10ng total RNA minimum recommended for optimal performance and FFPE samples
Mechanism of Action Bead-based rRNA depletion, cDNA synthesis, and PCR Enzymatic rRNA depletion, ligation-based addition of adapters and indexes
Method Whole-Transcriptome Sequencing Whole-Transcriptome Sequencing
Multiplexing High-throughput kits: Prepare 96 indexes samples using either combinatorial dual indexes or unique dual indexes (available soon), Low-throughput kits: Pool up to 12 samples. Or pool up to 24 samples with sets A and B together Up to 384 Unique Dual Indexes (UDIs)
Specialized Sample Types Blood FFPE Tissue, Low-Input Samples
Species Category Human, Mouse, Rat Bacteria, Human, Mouse, Rat, Virus
Species Details rRNA Removal: Depletes human, mouse, and rat rRNA (cytoplasmic and mitochondrial) and globin mRNA Ribo-Zero Plus included for depletion of abundant transcripts from multiple species including: human cytoplasmic & mitochondria rRNA, mouse rRNA, rat rRNA, bacteria Gram +/- rRNA, human beta globin transcripts
Technology Sequencing Sequencing

The product configuration for Cat. No. RS-122-2501, RS-122-2502, and RS-122-2503 has changed. In the new configuration, the library preparation and index adapters can be purchased separately. The list of components available for individual purchase can be found in the “Select Product(s)” section.

Method-Specific Workflow Example

 

Supporting Data and Figures

 

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Limited Use Label License: The TruSeq Stranded Total RNA with Ribo-Zero Globin Workflow product and its use are the subject of one or more issued and/or pending U.S. and foreign patent applications owned by Max Planck Gesellschaft, exclusively licensed to New England Biolabs, Inc. and sublicensed to Illumina, Inc. The purchase of this product from Illumina, Inc., its affiliates, or its authorized resellers and distributors conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product by the buyer (whether the buyer is an academic or for profit entity). The purchase of this product does not convey a license under any claims in the foregoing patents or patent applications direct to producing the product. The buyer cannot sell or otherwise transfer this product or its components to a third party or otherwise use the product for the following COMMERICAL PURPOSES: (I) use of the product or its components in manufacturing; or (2) use of the product or its components for therapeutic or prophylactic purposes in human or animals.