MeRIP-Seq/m6A-seq
MeRIP-Seq/m6A-seq
MeRIP-Seq maps m6A-methylated RNA. In this method, m6A-specific antibodies are used to immunoprecipitate RNA. RNA is reverse-transcribed to cDNA and sequenced. Deep sequencing provides high-resolution reads of m6A-methylated RNA.
Similar methods: miCLIP, m6A-LAIC-Seq.
Pros:
- Maps m6A methylated RNA
Cons:
- Antibodies not specific to target will precipitate nonspecific RNA modifications
- MeRIP-Seq: Meyer K. D., Saletore Y., Zumbo P., Elemento O., Mason C. E., et al. (2012) Comprehensive analysis of mRNA methylation reveals enrichment in 3' UTRs and near stop codons. Cell 149: 1635-1646
- m6A-Seq:Dominissini D. et al. (2012) Topology of the human and mouse m6A RNA methylomes revealed by m6A-seq. Nature 485: 201-206
- Gokhale N. S., McIntyre A. B., McFadden M. J., et al. N6-Methyladenosine in Flaviviridae Viral RNA Genomes Regulates Infection. Cell Host Microbe. 2016;
- Lin S., Choe J., Du P., Triboulet R. and Gregory R. I. The m(6)A Methyltransferase METTL3 Promotes Translation in Human Cancer Cells. Mol Cell. 2016;62:335-345
- Meng J., Lu Z., Liu H., et al. A protocol for RNA methylation differential analysis with MeRIP-Seq data and exomePeak R/Bioconductor package. Methods. 2014;69:274-281