Introducing the HiSeq™ Expert Video Tips series. This 5-part series includes usage tips, troubleshooting advice, best practices, and other common customer questions regarding the HiSeq instrument.

This video discusses how to choose the appropriate sequencing primers for your run. Since commonly used Illumina indexes, TruSeq™ and Nextera™, utilize different index primer site sequences, it’s important that the correct primer is loaded into the HiSeq for sequencing.

Presenters:
Chad Buckner, Field Service Engineer, Illumina
Tim Gilmartin, Staff Field Applications Scientist, Illumina

Related videos in series:
Instrument Computer Tips | HiSeq™ Expert Video Tips Series
youtu.be/gfN6bQdTZ90

Mechanical System, Thermal System, and Maintenance Best Practices | HiSeq™ Expert Video Tips Series
youtu.be/ha4x4LsseC0

Tips for Proper Fluidics Flow | HiSeq™ Expert Video Tips Series
youtu.be/JO3BksGkEt0

Removing Fluidic Line Blockages | HiSeq™ Expert Video Tips Series
youtu.be/UCWDDUjH7cQ

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Disclaimer: For Research Use Only. Not for use in diagnostic procedures.