Fastest, simplest. For every lab.
Remarkably simple from run setup to data analysis
The MiSeq i100 Series delivers our fastest run times yet, breakthrough simplicity, and significant sustainability advancements to empower every lab, everywhere
Same-day (and same-shift) results are now possible. The MiSeq i100 and MiSeq i100 Plus Systems provide results 4× faster than the MiSeq System, with sequencing run times as fast as four hours.
With the expanded output offered by the MiSeq i100 Series, you can increase sample throughput, perform deeper sequencing, and gain more insights for a wide range of applications faster than ever. XLEAP-SBS chemistry on the MiSeq i100 Series eliminates thawing requirements so reagents can be used whenever your sample is ready.
Every aspect of the MiSeq i100 Series workflow is noticeably simplified, from setup to data analysis. Whether you are new to sequencing or an experienced user, you’ll appreciate the streamlined operations. Run setup requires only three steps and can be completed in less than 20 minutes.
Integrated cartridges simplify library loading and eliminate the need for washes between runs, improving sequencing efficiency.
Generate data analysis summaries in two hours or less for most applications by accessing preconfigured DRAGEN pipelines, either onboard or in the cloud.
In addition to breakthrough advancements in system design, XLEAP-SBS chemistry, and integrated data analysis, the MiSeq i100 Series is optimized to reduce environmental impact. Key innovations, such as an 85% reduction in packaging waste compared to the MiSeq System and the use of recyclable plastics and room-temperature ship-and-store consumables, deliver remarkable benefits in terms of sustainability and user experience.
Trusted for over a decade, the MiSeq System has been shipped to over 10,000 customers globally, and has been cited in over 160,000 peer-reviewed publications.1
Building on our extensive expertise, Illumina has a relentless commitment to innovation and building future next-generation sequencing (NGS) capabilities and applications. The MiSeq i100 Series demonstrates our commitment to increasing access to genomics technology by continuing to deliver faster, simpler sequencing.
MiSeq i100 Series specificationsa
a. Specifications based on Illumina PhiX control library at supported cluster densities.
b. Maximum range based on 100M flow cell specifications. The 100M flow cell will be available starting in 2025 for the MiSeq i100 Plus System only.
See how the MiSeq i100 Series is setting the standard for simplicity, usability, and speed of benchtop sequencing from Illumina.
Illumina provides a world-class support team composed of experienced scientists who are experts in library prep, sequencing, and analysis. This exceptional technical support is available via phone five days a week or online 24/7, worldwide, and in multiple languages, with rapid response time near most major metropolitan areas.
To help you get high-quality results on Illumina technology even faster, we offer instructor-led or hands-on courses and web-based training options.
With both supported workflows and a large ecosystem of choices, Illumina products are available for every step of the MiSeq i100 Series workflow.
With the MiSeq i100 Series, libraries are loaded in three simple steps and in under 20 minutes.
Increase efficiency with XLEAP-SBS chemistry and streamlined operations, including onboard denaturation and clustering to obtain fast, high-quality data.
Obtain accurate and efficient secondary analysis with preconfigured DRAGEN pipelines onboard or in the cloud, generating results in two hours or less for most applications.
Gain insights and drive discovery with cloud-based tertiary analysis solutions for select workflows.
The MiSeq i100 Series offers highly accurate quality data for various applications, including oncology, infectious disease, and microbiology studies.
MiSeq i100 Seriesa |
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| Run time (range) | ~4–15.5 hr | ~5–56 hr | 8–44 hr |
| Max output | 30 Gb | 15 Gb | 540 Gb |
| Max reads per run | 100 million single reads |
25 million single reads 50 million paired-end reads |
1.8 billion single reads 3.6 billion paired-end reads |
| Informatics options | On-instrument: Custom menu of DRAGEN analysis pipelines Cloud: Broad menu of DRAGEN analysis applications available on BaseSpace Sequence Hub and Illumina Connected Analytics On-premises: Broad menu of DRAGEN analysis applications |
On-instrument: LRM analysis modules Cloud: Broad menu of DRAGEN analysis applications available on BaseSpace Sequence Hub and Illumina Connected Analytics On-premises: Broad menu of DRAGEN analysis applications |
On-instrument: Custom menu of DRAGEN analysis pipelines Cloud: Broad menu of DRAGEN analysis applications available on BaseSpace Sequence Hub and Illumina Connected Analytics On-premises: Broad menu of DRAGEN analysis applications |
a. Maximum range based on 100M flow cell specifications. The 100M flow cell will be available starting in 2025 for the MiSeq i100 Plus System only.
Watch the unveiling of the MiSeq i100 Series in this webinar. Discover how it will accelerate insights and discoveries with faster, simpler sequencing.
These resources cover key topics in NGS designed for beginners. We'll provide tutorials, guide you through the workflow and planning your first experiment.
Get up and running quickly with a rental or leasing program designed to work with your budget. Choose from several options to obtain financing, including instrument leasing, instrument rental, and reagent rental.
Illumina Proactive is a secure and remote instrument performance and support service. Our field support team can identify risks before your run, perform reagent lot tracking, maximize uptime, and improve lab efficiency. Register for a MyIllumina account to get started.
Reagents for the MiSeq i100 Series feature easy-to-use cartridges and multiple flow cell configurations for flexible sequencing options.
A fast, integrated workflow for preparing libraries for use in a wide range of sequencing applications.
A fast, integrated workflow for producing enriched and indexed sequencing libraries from a broad range of sample types and RNA input quantities.
A flexible and streamlined NGS library prep kit that enables various public health surveillance and microbial research applications.
Looking for more? Reach out to talk to a specialist about the MiSeq i100 Series or explore a variety of events introducing the MiSeq i100 Series, from roadshows in cities around the world to webinars and more.
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