Illumina Demonstrated 16S Protocol

Illumina Demonstrated 16S Protocol

Illumina Demonstrated 16S Protocol describes a method for preparing samples for sequencing the variable V3 and V4 regions of the 16S rRNA gene. This protocol can also be used for sequencing other regions with different region‐specific primers. This protocol combined with a bench top sequencing system, on‐board primary analysis, and secondary analysis using MiSeq Reporter or BaseSpace, provides a comprehensive workflow for 16S rRNA amplicon sequencing.

This protocol requires several user supplied consumables and at least one of the following Nextera XT Index kits:
FC-131-1001 Nextera XT Index Kit (24 indices, 96 samples)
FC-131-1002 Nextera XT Index Kit (96 indices, 384 samples)
FC-131-2001 Nextera XT Index Kit v2 Set A (96 indices, 384 samples)
FC-131-2002 Nextera XT Index Kit v2 Set B (96 indices, 384 samples)
FC-131-2003 Nextera XT Index Kit v2 Set C (96 indices, 384 samples)
FC-131-2004 Nextera XT Index Kit v2 Set D (96 indices, 384 samples)
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* Library prep is compatible with all Illumina instruments. Recommended instruments chosen based on typical output and analysis need for selected application.
† Number of samples per run depends on sample diversity.
Project Recommendations
Species Compatibility Most prokaryotic microbial
Recommended Illumina Instrument* MiSeq
Recommended Samples per run† Up to 384
Max Recommended Read Length 2x300 bp
Recommended Secondary Analysis
  • MiSeq Reporter 16S Metagenomics Workflow
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For Research Use Only

Not for use in diagnostic procedures (except as specifically noted).

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