Nextera DNA Flex provides a streamlined, robust, and automation-ready workflow process developed for use on all Illumina sequencers, that delivers high-quality, highly reproducible libraries with consistent performance across a variety of genomes. The Flex Lysis Reagent kit (optional) is to process blood or saliva samples.
One of the following Nextera Index kits is needed, even if multiplexing is not used (these oligos are used in PCR and attached your library to the flow cell) Catalog ID(s):
| Species compatibility | Compatible with most large DNA genomes | ||||
|---|---|---|---|---|---|
| Recommended Illumina instrument | NextSeq 500 | HiSeq 2500 (Dual Flow Cell) | |||
| Recommended samples per run and chemistry version (eg, 24 on v3)† | 1 | 2–10 | |||
| Run mode | High Output | Rapid run mode | High Output | ||
| Max recommended read length | Up to 2 × 150 bp | Up to 2 × 125 | Up to 2 × 150 | ||
| Recommended secondary analysis (eg, MiSeq Reporter, Basespace) | BWA WGS BaseSpace App Issac: WGS v2 BaseSpace App SPAdes Genome Assembler |
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| Recommended down stream analysis (eg, VariantStudio) | Variant Studio | ||||
* Library prep is compatible with all Illumina instruments. Recommended instruments chosen based on typical output and analysis need for selected application.
† Based on 30× coverage of a human genome.