The TruSeq Methyl Capture EPIC Library Prep Kit includes nearly all the reagents needed to prepare targeted Methyl-Seq libraries for NGS. With a content design driven by customer-based requests and emerging regions of interest identified by ENCODE, FANTOM5, and the Epigenomics RoadMap Consortium, the TruSeq Methyl Capture EPIC Library Prep Kit spans the full human methylome. For unbiased target enrichment during library preparation, the target capture step is performed before bisulfite conversion.
| Species Compatibility | Human | |||||
|---|---|---|---|---|---|---|
| Recommended Illumina Instruments | NovaSeq 6000 (Dual Flow Cell)† | HiSeq 3000/4000 | HiSeq 2500 | NextSeq 500/550 | ||
| Recommended Samples per run and chemistry version (i.e.: 24 on v3)‡ | 48 | 48 | 96 | 3000: 48 4000: 96 |
Rapid Run: 8–12 High Output v4: 64 |
High Output: 8 |
| Run Mode | S1§ | S2 | S4 | 3000, Single Flow Cell 4000, Dual Flow Cell |
Dual Flow Cell | High Output |
| Max Recommended Read Length | 2×100 bp | 2×100 bp | 2×100 bp | 2×100 bp | ||
| Recommended Secondary Analysis (i.e. MiSeq Reporter, Basespace) | BaseSpace Apps MethylSeqv2 and MethylKitv2 | |||||
* Library prep is compatible with all Illumina instruments. Recommended instruments chosen based on typical output and analysis need for selected application.
† Limited by available index combinations. Based on >55M reads.
‡ Based on samples at 40× mean coverage and >90% of target bases are covered at ≥10×.
§ NovaSeq S1 Reagent Kits are not currently available.
| Total Size | 107 Mb |
|---|---|
| Number of CpG sites | 3.34M |
| Transcription Factor Binding Sites | 180K |
| FANTOM5 Enhancers | 28.2K |