TruSeq Nano DNA

TruSeq Nano DNA
LT HT

Simple, all-inclusive prep solution, ideal for whole-genome sequencing. This new workflow offers reduced DNA input allowing for interrogation of samples with limited available DNA while providing excellent genomic coverage and uniformity. The LT option is available in Set A and Set B. Each set contains 12 unique single indexes that can be pooled together for up to 24plex.

Simple, all-inclusive prep solution, ideal for whole-genome sequencing. This new workflow offers reduced DNA input allowing for interrogation of samples with limited available DNA while providing excellent genomic coverage and uniformity. The HT option provides dual index combinations for up to 96plex.

LT Catalog ID(s):
FC-121-4001 (12 Set A index tubes, 24 samples)
FC-121-4002 (12 Set B index tubes, 24 samples)
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HT Catalog ID(s):
FC-121-4003 (96 indices in plate format, 96 samples)
The index plate is single use per well. Do not undergo more than four freeze/thaw cycles. We strongly recommend processing at least 24 samples at a time
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Project Recommendations
Species Compatibility Compatible with most large DNA genomes
Recommended Illumina Instrument* NextSeq 500 HiSeq 2500 (Dual Flow Cell) NovaSeq 6000 (Dual Flow Cell)
Recommended Samples per run† 1 2–10 8 16 48
Run Mode Mid Output High Output Rapid Run High Output S1‡ S2 S4
Max Recommended Read Length 350 bp insert: ≤ 2×101 bp
550 bp insert: ≤ 2×151 bp		 350 bp insert: ≤ 2×101 bp
550 bp insert: ≤ 2×151 bp		 350 bp insert: ≤ 2x101 bp
550 bp insert: ≤ 2x151 bp		 350 bp insert: ≤ 2x101 bp
550 bp insert: ≤ 2x126 bp		 350 bp insert: ≤ 2×150 bp
550 bp insert: ≤ 2×150 bp
Recommended Secondary Analysis
  • BWA WGS BaseSpace App
  • Isaac WGS v2 BaseSpace App
  • SPAdes Genome Assembler
Recommended Down Stream Analysis VariantStudio

* Library prep is compatible with all Illumina instruments. Recommended instruments chosen based on typical output and analysis need for selected application.
† Based on 30x coverage of a human genome.
‡ NovaSeq S1 Reagent Kits are not currently available.

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For Research Use Only

Not for use in diagnostic procedures (except as specifically noted).

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