TruSeq Small RNA
TruSeq Small RNA
TruSeq small RNA enables library preparation of any miRNA with a 3’ hydroxyl and a 5’ monophosphate, like those processed by Dicer/Drosha. Compatible applications include finding novel miRNAs, characterizing variation such as isomer with single-base resolution, and analyzing differential expression without prior assumptions.
Starting Material: Optimized for 1ug high-quality total RNA, or previously isolated miRNA from 1-10ug total RNA (likely 10-50ng miRNA).
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| Species Compatibility | Any Species (captures small RNA with 3′ OH and 5′P modifications) | ||||||
|---|---|---|---|---|---|---|---|
| Recommended Illumina Instrument* | MiniSeq | MiSeq | NextSeq | ||||
| Recommended Samples per run† | 1 to 2 (based on 5M read) | 5 (based on 5M reads/sample) | 3 to 5 | 26 to 48 | |||
| Run Mode | Mid Output | High Output | – | Mid Output | High Output | ||
| Max Recommended Read Length | 1×50 bp | 1×50 bp | 1×50 bp | 1×50 bp | 1×50 bp | ||
| Recommended Secondary Analysis |
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MiSeq Reporter Small RNA Workflow | FASTQ files with 3rd party analysis | ||||
*Library prep is compatible with all Illumina instruments. Recommended instruments chosen based on typical output and analysis need for selected application.
†Based on 5M reads per sample.